alexa Effect of smoking and microorganicms on Human Gingival Fibroblasts

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Effect of smoking and microorganicms on Human Gingival Fibroblasts

Monocyte chemoattractant protein-1 (MCP-1) is an inducible protein that attracts monocytes to areas of injury and infection. Studies have shown that it is produced by gingival cells in periodontal diseases and that its incidence increases with the severity of the disease. The aim of the present study was to investigate the effects of nicotine, cigarette smoke condensate (CSC), and Porphyromonas gingivalis (P.  gingivalis) on MCP-1 expression from human gingival fibroblasts (HGFs) in vitro.HGFs were exposed for 72 h to 250 µg/mL of nicotine, 100 µg/mL of CSC, 10% P. gingivalis supernatant, P. gingivalis supernatant with nicotine, or P. gingivalis supernatant with CSC. A control group comprised HGFs without any treatment. The conditioned media was then collected for MCP-1 analysis by enzyme-linked immunosorbent assay (ELISA).
There were significant differences in MCP-1 level between the P. gingivalis (p=0.0432) and P. gingivalis with CSC (p=0.0037) groups when compared to the control group.
P. gingivalis stimulates an inflammatory response in periodontal tissues by increasing MCP-1, which helps attract host cells to combat the bacterial infection. Tobacco usage can mask the inflammatory responses normally seen in periodontal diseases by reducing the levels of MCP-1, thus allowing the bacteria to grow somewhat undetected. This could be one factor that explains why smoking is a major contributing factor to the initiation, development, and progression of periodontal diseases.

 

Reference: Eman Allam, Mia Recupito, Hend Mohamed, L. Jack Windsor (2015) In Vitro Effects of Nicotine, Cigarette Smoke Condensate, and Porphyromonas gingivalis on Monocyte Chemoattractant Protein-1 Expression from Cultured Human Gingival Fibroblasts. J Interdiscipl Med Dent Sci 3:171. doi: 10.4172/2376-032X.1000171

  • Share this page
  • Facebook
  • Twitter
  • LinkedIn
  • Google+
  • Pinterest
  • Blogger
Top