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Understanding however protozoal infection parasites move between populations is vital, significantly given the potential for protozoal infection to be reintroduced into areas wherever it had been antecedently eliminated. we tend to examine the distribution of protozoal infection biology across seven sites among the Democratic Republic of Congo (DRC) and 2 close countries, Republic of Ghana and African nation, so as to know however the connection of protozoal infection parasites varies across house, and whether or not there area unit barriers to the flow of protozoal infection parasites among the DRC or across borders. Parasite de oxy ribonucleic acid was retrieved from dried blood spots from seven Demographic and Health Survey sample clusters within the DRC. Protozoal infection genetic characteristics of parasites from Republic of Ghana and African nation were additionally obtained.