In order to isolate xylanolytic microbial strains, screening and isolation was done using agricultural waste and decaying biomass. The enzyme super-secreter Aspergillus flavus MTCC 9390 was selected for optimized production of xylanase. Various process variables were optimized using conventional ‘one-variable-at-a-time’ approach which involves varying a single independent variable and maintaining others at a constant level. All culture conditional variables had profound influence on enzyme production and 15-30% increase was brought by nitrogen source only. A synergistic five-fold increase in xylanase production was achieved when an inoculums size of 2 x 106 spores/ mL was incubated in modified Czapek Dox-A for 6 days at pH 6.0 and temperature 45ºC under static conditions in submerged fermentation.
Citation: Bhushan B, Pal A, Jain V (2012) Isolation, Screening and Optimized Production of Extracellular Xylanase under Submerged Condition from Aspergillus Flavus Mtcc 9390. Enzyme Engg 1:103. doi: 10.4172/2329-6674.1000103