Partition of Pepsinogen from the Stomach of Red Perch

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Partition of Pepsinogen from the Stomach of Red Perch

Fish processing waste can be used to produce commercially valuable by-products, such as pepsinogen, which has application in food, manufacturing industries, collagen extraction, gelatin extraction, and in regulating digestibility.An important acidic protease, pepsin, is synthesized and secreted in the gastric membrane in an inactive state called pepsinogen (PG). In the present study, the purification of pepsinogen from the stomach of red perch, using aqueous two phase systems (ATPS) formed by polyethylene glycol (PEG) and salt at 4°C, was optimized.The effects of PEG molecular weight (PEG 1000, 1500, 3000 and 4000) and concentration (16, 18, 20, 22 and 24%) on the partitioning of PG were studied, and parameters including total volume (TV), volume ratio (VR), total enzyme activity (AE), protein content (Cp), specific enzyme activity (SA), partition coefficient (Kp), purification fold (PF), and recovery yield (RY) were evaluated.

Citation: Zhao L, Budge SM, Ghaly AE, Brooks MS, Proces DD (2013) Partition of Pepsinogen from the Stomach of Red Perch (Sebastes marinus) by Aqueous Two Phase Systems: Effects of PEG Molecular Weight and Concentration. Enz Eng 2:108.

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