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The aim of this work was to investigate the ability of silicate wire ion selective electrode to measure 2,4 dichlorophenoxyacetic acid (2,4-D). The slope of the electrode was 56.2 mV decade -1 in the range from 10-6 to 10-2 M with lower limit of detection 8.3 × 10-7 M. The electrodes were successfully applied to determination of 2,4-D by separate and standard addition potentiometry. The electrode was used to detect the effect of pH and temperature on 2,4-D placed on baby watercress plant grown in the clay soil and the residue after five day from addition.