It is very important to select a proper freezing temperature and cryopreservation solution to minimize tissue damage during the freezing process. Animal serum, culture media, and dimethyl sulfoxide (DMSO) have been used as cryopreservation solutions, and methods have been develop to slowly decrease temperatures during the freezing process to minimize tissue damage. There is a need for rapid thawing to isolate cells from cryopreserved tissue or to reuse cryopreserved tissue.
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Last date updated on July, 2014