Over the last 20 years, a number of methods have been developed to confine the architecture of cultured neuronal networks including microfluidics, topographical cues, microstamping, collagen, organosilanes, or alkanethiols. Geometric patterns created include simple lines, 4 nodes and 8 node interconnected grids, loops, and even crude logic devices. The engineered geometry of these neuronal networks has been used for the study of plasticity as well as a number of biosensor applications. Although a wide variety of methods exist to create desired network geometries, an effective means to engineer neuronal polarity within those geometries has not been established.
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Last date updated on November, 2020