Agar was chosen to serve as a semi-solid matrix to embed bacteria uniformly throughout a fixed area. Since agar is also a polysaccharide polymer, it also provides a three-dimension barrier for the microorganisms embedded within. The agar medium served as a simple way to assess nonthermal plasma penetrative efficacy, through biological polymers. Since the surrounding medium plays a role in how the plasma is able to interact with target cells, penetrative efficacy must be considered.
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Last date updated on July, 2014