The term shoot culture is now preferred for cultures started from explants bearing an intact shoot meristem, whose purpose is shoot multiplication by the repeated formation of axillary branches. In this technique, newly formed shoots or shoot bases serve as explants for repeated proliferation; severed shoots, explant size, shoot cultures are conventionally started from the apices of lateral or main shoots, up to 20 mm in length, dissected from actively-growing shoots or dormant buds. Larger explants are also sometimes used with advantage: they may consist of a larger part of the shoot apex or be stemming segments bearing one or more lateral buds; sometimes shoots from other in vitro cultures are employed. When apical or lateral buds were used almost exclusively as explants, the name shoot tip culture came to be widely used for cultures of this kind.
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Last date updated on July, 2014