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RFLPs based molecular markers are amongst the first generation molecular markers used for many animal and plant genome mapping projects.This technique is based on digesting genomic DNA with a certain restriction enzymes and followed by southern blotting.Due to mutation there is a chance of gain or loss of restriction sites. As a result when homologous chromosomes are digested with restriction enzymes, electrophoresis and probed fragment length polymorphism is obtained. RFLPs are so sensitive that it can detect single- nucleotide mutations, deletions or insertions of DNA fragments of very small number of base pairs (1-100bp). Rearrangements of DNA on large chromosomal fragments can also be detected by RFLPs. RFLPs are well known for its robustness and accuracy in genotyping.
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