|Technological development for the diagnosis of HIV infection during over two decades, has favored the incorporation of modifications in the Western blot assays (WB) that are routinely used as confirmatory tests. Double reactive samples to HIV-1/2 revealed the characteristic pattern of positive reactivity to HIV-1 and a high signal in the band of peptide, which showed that solid phase with the incorporated peptide, is not only capable of discriminating between the two infections, but also it has the additional attribute to diagnose a possible double infection by both viruses. DAVIH-BLOT HIV-1/2 system reached satisfactory results on performance indicators studied in front of the reference panel and in the comparative study with the commercial kit of similar principle
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