Quantification of Pharmacologically Active Marker Gallic Acid and Ellagic Acid from Leaf and Stem of Pergularia daemia Forsk. by HPTLC MethodSutar NG1,2 and Pal SC3*
- *Corresponding Author:
- Pal SC
Department of Pharmacognosy
RG Sapkal College of Pharmacy
Anjeneri, Nasik, Maharashtra, India
E-mail: [email protected]
Received Date: October 19, 2015; Accepted Date: December 09, 2015; Published Date: December 09, 2015
Citation: Sutar NG, Pal SC (2015) Quantification of Pharmacologically Active Marker Gallic Acid and Ellagic Acid from Leaf and Stem of Pergularia daemia Forsk. by HPTLC Method. J Anal Bioanal Tech 7:291. doi:10.4172/2155-9872.1000291
Copyright: © 2015 Sutar NG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
TLC densitometry method for quantification of gallic acid and ellagic acid using HPTLC is developed. This is the first report of quantification of these two biologically active compounds as gallic acid and ellagic acid using HPTLC from this plant. Quantification of gallic acid and ellagic acid was carried out from the methanol extract by using solvent system of Toluene: Ethyl acetate: Formic acid (6:4:0.8 v/v/v). The Rf value of Gallic acid and ellagic acid are 0.43 and 0.38 respectively. The linearity ranges of gallic acid (100 to 600 ng) and ellagic acid (100 to 600 ng) with correlation coefficient (r- values) of 0.9994 and 0.9997 respectively. The amount of gallic acid and ellagic acid was 3.01 and 11.09 μg/ml in leaf and 4.97 and 18.30 μg/ml in stem respectively. Quantification of gallic acid and ellagic acid showed good separation and resolution from other constituent of extract. Its main advantages are its simplicity, accuracy and selectivity. This method can also be used for estimation of these compounds in other herbal preparation and may be useful for standardization purposes.