Abstract

To assess the impact of popular American cigarette flavouring components on tumour promotion, four comparative twostage SENCAR mouse skin painting bioassays were carried out using cigarette smoke condensate (CSC) preparations. Each independent study made use of a different flavouring combination that was added to tobacco at heightened levels, and the evaluation of 150 ingredients as a whole. A topically applied dose of 50 g of 7,12-dimethylbenz(a)anthracene (DMBA) was used to commence each group of 30–50 female SENCAR mice, and either 10 or 20 mg of CSC from test cigarettes containing component mixes was used to encourage the animals every three weeks for 26 weeks. For comparison, several mouse groups were treated concurrently with CSC from reference cigarettes that had no other components. Acetone was used as a negative control, and 12-o-tetradecanoyl-phorbol-13-acetate (TPA) was used as a positive control. CSC-exclusive groups.