Received Date: May 08, 2013; Accepted Date: June 12, 2013; Published Date: June 14, 2013
Citation: Li W, Chen G, Yu X, Shi Y, Peng M, et al. (2013) Accumulation of the Mutations in Basal Core Promoter of Hepatitis B Virus Subgenotype C1 Increase the Risk of Hepatocellular Carcinoma in Southern China. J Clin Exp Pathol 3:141. doi: 10.4172/2161-0681.1000141
Copyright: © 2013 Li W, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Visit for more related articles at Journal of Clinical & Experimental Pathology
Background: Hepatitis B virus (HBV) genotype C is associated with the development of Hepato Cellular Carcinoma (HCC). In addition, HBV subgenotype C1 is the major subgenotype in Southern China. The aim of this study was to investigate whether there was the specific mutation patterns in HBV/C1 associated with Southern Chinese patients with HCC.
Methods: Mutations in HBV Basal Core Promoter (BCP) and their association with HCC were assessed in a matched cross-sectional control study of 102 HCC and 105 Chronic Hepatitis (CH) patients (from Guangdong, China) infected with HBV/C1. Functional analysis of HBx mutants was performed by the colony formation assay and the luciferase assays.
Results: T1762/A1764 double mutations was frequently found in patients infected with HBV/C1, regardless of clinical status (64.7% in HCC and 51.4% in CH, P>0.05). Unexpectedly, the adjacent V1753 or A1768 mutation significantly increased the risk of HCC (P<0.05). Moreover, the prevalence of triple or quadruple mutations in BCP was significantly higher in patients with HCC than those with CH, particularly for HBeAg-positive-carriers (P<0.05). Functional analysis revealed that T1762/A1764 mutation alone did not alter the transcriptional activity and the inhibitory effects on cell proliferation and apoptosis of HBx, but triple or quadruple mutations largely abrogated this effect.
Conclusions: Accumulation of mutations involving V1753 or/and A1768 in addition to T1762/A1764 in BCP region were closely related to HCC among the patients infected with HBV/C1, particularly for HBeAg-positive-carriers. The increased risk of HCC caused by BCP variants may be attributable partially to modifying the biological functions of HBx.
Hepatitis B virus; Basal core promoter; X gene; Mutation; Hepatocellular carcinoma
Hepato Cellular Carcinoma (HCC) is the fifth most frequent cancer and the third leading cause of cancer-related death in the world . It is accepted that chronic infection with Hepatitis B virus (HBV) is a major risk factor for the development of HCC . The HBV genome consists of a partially double-stranded circular DNA molecule comprising 3200 nucleotides, and exhibits considerable genetic variability because the polymerase lacks a proofreading function. At present, HBV is classified into eight genotypes (A to H) according to a divergence in the entire nucleotide sequence greater than 8% . HBV genotypes have distinct geographical distributions and correlate with the severity of liver disease [3,4]. Genotypes B and C are prevalent in Asia, and genotype C is associated with a more severe course of liver disease and a higher risk of HCC development than genotype B [5,6]. Furthermore, subgenotypes have been identified in different HBV genotypes based on a divergence greater than 4% but less than 8% in the complete nucleotide sequence. There are two subgenotypes of genotype B with distinct geographical distributions, provisionally designated B1 (Bj, Japan) and B2 (Ba, other Asian countries) . Clinical differences between the patients infected with HBV/Ba and HBV/Bj have been demonstrated, indicating a comparatively better prognosis of HBV/Bjinfection in comparison to HBV/Ba [8,9]. Recently, HBV/C has been classified into four subgenotypes (HBV/C1-C4) [10,11]. The two most widely spread subgenotypes are HBV/C1 (Cs), for Southeast Asia, and HBV/C2 (Ce), for East Asia; they not only have different geographic distributions but also different clinical outcomes are becoming clear [12,13].
The HBV Core Promoter (CP) resides in the overlapping X gene and plays a central role in HBV replication and morphogenesis . Core promoter is composed of the core upstream regulatory sequence (CURS) and the Basal Core Promoter (BCP). Mutations in BCP region at nucleotides (nt) 1762 and 1764 (T1762/A1764) were previously reported in association with HBe antigen seroconversion and viral replication. They were often found in the patients with advanced liver disease and HCC [15-19]. Recently, T1653 mutation in the box α of CURS and V1753 mutation in BCP region has also been reported to increase the risk of HCC in HBeAg-negative patients infected with HBV/C [20,21]. As well, specific mutations in CP region of HBV were differently associated with HCC in the context of HBeAg status among HBV/C1 and HBV/C2 carriers . However, the mechanism underlying CP mutation and hepatocarcinogenesis is largely unknown.
As a highly endemic area for HBV infection, the prevalence of chronic HBV infection in China is between 8 and 20% of the general population, with an estimated 120 million hepatitis B surface antigen (HBsAg) carriers. The prevalence of HBV is uneven in different regions of China: high in some southern provinces (>8%) and low in some western provinces (4–6%) . Genotypes B and C were identified as the most common HBV strains and account for approximately 95% of Chinese patients. Further study confirmed all genotype B strains belong to subgenotype Ba. Two of genotype C subgenotypes, C1 and C2, were found in China and they showed different geographic distributions. HBV/C1 was the major subgenotype in Southern China and C2 was predominant in Northern China [23-26]. Previous reports indicated a significantly higher incidence of HCC in Southern China [27,28]. However, it is unclear whether there are the specific mutation patterns in CP of HBV responsible for the development of HCC in Southern China.
The aim of this study was to determine the HCC-associated mutations of the HBV genome in the entire CP and the overlapping X gene region in Southern Chinese patients infected with HBV/C1, and analyze the hotspot mutations through a cross-sectional control study.
A total of 209 serum samples were obtained from a cohort of patients with chronic hepatitis B, including 102 from Chronic Hepatitis (CH) patients and 102 from HCC patients, who had been recruited at the First affiliated Hospital of Guangzhou Medical College, Guangzhou China between June 2005 and December 2009. The diagnosis of HCC was made pathologically. All patients were chronic HBV C1 carriers, as identified by PCR-restriction fragment length polymorphism. None of the patients had co-infection with hepatitis C virus or other agents that are causes of liver disease. All serum samples were stored at -40°C until analysis.
Amplification and sequencing of CP region of HBV
HBV DNA was extracted from 100 µl serum using a QIAamp DNA blood mini kit (QIAGEN GmbH, Hilden, Germany) according to the manufacturer’s instructions. In order to detect the genetic variations in CP region, HBV DNA sequence (nt. 1176-1838) bearing the core promoter region and the overlapping X gene was amplified by PCR with hemi-nested primers. The first-round PCR was performed with the sense primer HBVF (1176-1195) and the antisense primer HBVR (1823-1804). The second-round PCR was performed with the sense primer HBVF (1266-1285) and the antisense primer HBVR (1823- 1804) (Table 1). Amplified products were directly sequenced with Prism BigDye (Applied Biosystems, Foster City, CA) in the ABI 3100 DNA automated sequencer. Sequences were compared using the Clustal W program.
Site-directed mutagenesis and plasmid construction
The mutations in CP region were introduced by overlap extension PCR, by PCR-mediated site-directed mutagenesis with a set of designed mutagenetic primers (Table 1), as reported previously . Briefly, single base changes were introduced by PCR using internal primers containing the desired point mutation(s): HBVF2–2 (1739-1773), HBVF2–3.1 (1739-1773), HBVF2–3.2 (1739-1773) and HBVF2–4 (1739-1773), paired with the outside primer HBV1883R2. Primer set HBVF1 (1374-1393)/HBVR1 (1728-1756) was used to generate the products with the homologue ends overlapped with the above mutated fragments. The products of the first round PCR were purified and served as the templates for the second round PCR with the outside primer set HBVF1(1374-1393)/HBVR2(1813-1838) to generate a fulllength HBx. The PCR products were cloned into the HindIII-XhoI sites of pcDNA3.1 (Invitrogen, Carlsbad, CA) to obtain the expression vectors of wild type or mutated HBx, respectively. All constructs were sequenced to confirm the desired sequences.
The underlined letters, restriction enzyme site (HindIII and XhoI); the solid letters, X gene mutant sites.
Table 1: Oligonucleotide primers used in the study.
The NF-κB luciferase reporter plasmids, NF-κB-Luc and mtNF- κB-Luc have been previously described . NF-κB-Luc encodes consensus NF-κB sites upstream of a luciferase reporter; mtNF-κB-Luc is identical to NF-κB-Luc except that the NF-κB consensus sites are mutated so that NF-κB cannot bind and stimulate transcription.
Cell culture and transfection
HepG2 cells were maintained in Dulbecco’s modified Eagle’s medium (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Invitrogen) at 37°C in a 5% CO2 environment. For transfection, cells were seeded in 96-well plates at 1.5×105 cells/ ml, grown to 50-80% confluency and transfected with the plasmids described above using Lipofectamine2000 (Invitrogen) according to the manufacturer’s protocol. Following transfection, cells were incubated for 24 h before being harvested for the luciferase assay or gene expression assay.
Total cell lysates were prepared in RIPA buffer [50 mmol/L TrisHCl, pH 8.0, 150 mmol/L NaCl, 1% (vol/vol) Nonidet P-40, 0.5% (wt/vol) sodium desoxycholate, 0.1% (wt/vol) SDS] containing the complete protease inhibitor cocktail. Western blot analysis was performed as described with the following primary antibodies: monoclonal mouse anti-HBx (Abcam, CA, USA) and mouse anti-ß-actin (Sigma, MO, USA). Experiments were repeated in triplicate.
The Renilla luciferase reporter control plasmid, pRL-CMV and the pGL3 promoter luciferase reporter plasmid were purchased from Promega Corp (Madison, WI, USA). To analyze the NF-κB promoter activity, HepG2 cells grown in 24-well plates were co-transfected with expression vector (different forms of HBx or control plasmid) and reporter plasmid using Lipofectamine2000 (Invitrogen). At 48 hours after transfection, cells were harvested and assayed using Dual Luciferase Reporter Kit (Promega, CA, USA) as previously described .
After cells were transfected for 48 h, they were transferred to a 60 mm plate and cultured in G418 (Invitrogen) selective medium for 2 weeks (G418 700 µg/ml). The cell culture medium was changed every 3 or 4 days until the colonies formed. Drug-resistant colonies were fixed with cold methanol, stained with Giemsa, and then scored.
Cell apoptosis was assessed by flow cytometry using a PE Annexin V Apoptosis Detection Kit I (BD Pharmingen, San Diego, USA) and analyzed by BD FACSAriaTM Flow Cytometer and Modfit LT 3.0 (Verity Software House, Topsham, USA).
Mutations between the two groups of samples were analyzed with relative risk test (OR value) and chi-square test (χ2 analysis). Statistical comparisons between other indexes were analyzed by Student’s t-test. Regression and correlation were done by Pearson method. A P-value of less than 0.05 was taken as statistically significant.
Mutations in the core promoter of HBV/C1 in patients with HCC and CH
In order to detect the genetic variations in CP region, DNA fragment from HBV nt 1176-1838 was amplified by hemi-nested PCR. As expected, the most frequently occurring mutation in CP was T1762/ A1764, which could be found in 57.9% of all HBV isolates. However, there was no difference in the prevalence of T1762/A1764 between patients with HCC and CH (64.7% vs. 51.4%, P>0.05). Interestingly, the adjacent T1766 and A1768 mutations in BCP were significantly higher for the HCC group than for the CH group (18.6% vs. 6.7% and 24.5% vs. 8.6%, P<0.05, respectively) (Table 2).
|Mutation||Total (n=207)||HCC (n=102)||CH (n=105)||P value||OR (95% CI)|
|T1653||27 (13.0%)||18 (17.6%)||9 (8.5%)||0.088|
|V1753||43 (29.5%)||43 (42.1%)*||18 (17.1%)||0.034||3.071 (1.68–5.86)|
|T1762/A1764||120 (57.9%)||66 (64.7%)||54 (51.4%)||0.802|
|T1766||26 (25.5%)||19 (18.6%)*||7 (6.7%)||0.044||2.146 (0.96–7.12)|
|A1768||35 (16.9%)||26 (24.5%)*||9 (8.6%)||0.021||2.869 (1.21–6.49)|
HCC: Hepatocellular Carcinoma; CH: Chronic Heptitis; OR: Odds Ratio; CI: Confidence Interval
*P<0.05 comparing the frequency of CP mutation in HCC vs.CH.
Table 2: Prevalence of mutations in the core promoter region between HCC and CH patients infected with HBV/C1.
patients with HCC and CH (64.7% vs. 51.4%, P>0.05). Interestingly, the adjacent T1766 and A1768 mutations in BCP were significantly higher for the HCC group than for the CH group (18.6% vs. 6.7% and 24.5% vs. 8.6%, P<0.05, respectively) (Table 2).
The patterns of BCP mutation in patients infected with HBV/C1 are presented in Table 3. Single mutation was rare, which was only found in two cases for V1753. Interestingly, the prevalence of V1753 or A1768 mutation always occurred along with T1762/A1764. Although previously reports that T1762/A1764 double mutation increased the risk of HCC[4-7], no the correlation was observed between T1762/A1764 mutation and clinical diagnosis in this study. However, mutations at position 1762/1764 in addition to V1753 or A1768 exhibited a close association to HCC. The frequencies of 1753/1762/1764, 1762/1764/1768, or 1753/1762/1764/1768 mutations were significantly higher in patients with HCC than those with CH (11.8% vs.3.8%, 6.9% vs. 2.9%, or 2.9% vs. 0.95%, P<0.05, respectively).
|Type||Patterns||HCC (n=102)||CH (n=105)||P value|
|single mutation||-||0 (0%)||(23.8%)|
|double mutation||+||-||-||-||-||66 (64.7%)
HCC: Hepatocellular Carcinoma; CH: Chronic Heptitis *P<0.05 comparing the frequency of triple mutation or quadruple mutation in HCC vs.CH.
Table 3: Mutation patterns of BCP in HBV isolated from patients infected with HBV/C1.
Accumulation of BCP mutations and HBeAg status in patients with HCC and CH
BCP mutations were reported to be able to down-regulate the expression of HBeAg in cell culture system . We have compared the rates of accumulation of BCP mutations and HBeAg status in patients with HCC and CH (Table 4). Among HBeAg-positive patients, there was no difference in the rate of T1762/A1764 double mutation between HCC group and CH group (65.7% vs. 54.1%, P>0.05). However, the rates of accumulative BCP mutations, such as 1753/1762/1764, 1762/1764/1768, or 1753/1762/1764/1768 mutations were significantly higher for the HCC group than for the CH group (14.3% vs.4.1%, 8.6% vs. 2.7%, or 4.3% vs. 1.4%, P<0.05, respectively).
|Mutation||HBeAg-positive group||HBeAg-negative group|
|HCC (n=70)||CH (n=74)||P value||HCC (n=32)||CH (n=31)||P value|
|T1762/A1764/ A1768||10||0.027||2 (6.3%)||0.961|
|V1753/||(14.3%)*||3 (4.1%)||0.031||1 (3.1%)||1|
|(4.3%)*||1 (1.4%)||0 (0%)|
*HCC vs.CH, P < 0.05 (Yates corrected chi-square). Significant data are shown in bold
Table 4: Accumulation of BCP mutations among patients with HCC and CH in context of HBeAg.
Effect of BCP mutations on transactivation activity of HBx
BCP mutation V1753, T1762, A1764 and A1768 result in four amino acid (aa) substitutions in HBx protein, i.e., Isoleucine to asparagine/ serine/threonine, lysine-to-methionine, valine-to-isoleucine, and phenylalanine-to-tyrosine at aa 127, 130–132. T1766 does not cause the amino acid change (Figure 1). In order to define the biological impact of BCP mutations on HBx, we introduced a series of artificial mutants into a wild type HBx template. pcDNA3.1-Xwt and pcDNA3.1-Xmt-2, pcDNA3.1-Xmt-3.1, pcDNA3.1-Xmt-3.2 and pcDNA3.1-Xmt-4 were constructed to express wild type HBx and HBx mutants. Transfection elliency was monitored by comparing the relative amount of cellular X protein using Western blot analysis (Figure 2A). To monitor the transactivation activity of different HBx constructs, a luciferase assay using the reporter construct of NF-κB-Luc or mtNF-κB-Luc was performed. HepG2 cells were transiently cotransfected with HBx and NF-κB-Luc or mtNF-κB-Luc plasmids. Like the wild type HBx, 1762/1764 double mutation induced a 4-5-fold increase in luciferase activity, but 1753/1762/1764, 1762/1764/1768 or 1753/1762/1764/1768 mutations revealed largely inhibition of transactivation activity (Figure 2B). In contrast, we found that none of HBx constructs could activate mtNF-κB-Luc (Figure 2B). This result indicated that accumulative BCP mutations could inhibit the transcriptional activity of HBx.
Figure 2: Impact of HBx mutant on transactivation activity. A, expression of HBx and artificial mutants constructed according to the naturally occurring mutation pattern of BCP in HepG2 cells 48 h after transfection. B, transactivation activity of HepG2 cotransfected with NF-κB-Luc or mtNF-κB-Luc construct plus the indicated HBx artificial mutant or empty vector pcDNA3.1. Xwt: expression vector containing wild-type X gene; Xmt-2, Xmt-3.1, Xmt-3.2 and Xmt-4: expression vector containing mutants X gene, bearing T1762/A1764 double mutant, V1753/A1764/T1762 triple mutant, T1762/A1764/A1768 triple mutant and V1753/T1762/A1764/A1768 quadruple mutant, respectively. *P<0.05; **P<0.01 vs. controls, such as pcDNA3.1 empty vector.
Effects of BCP mutations on growth-suppressive effect of HBx
We then investigated the effects of HBx and artificial mutants on cell growth using a colony formation assay. HepG2 cells were transfected with the different HBx expression plasmids, and 2 weeks after G418 selection, the number of colonies was scored. As expected, the expression of HBx caused a substantial reduction (5-fold) in the number of colonies compared with the control cells transfected with the empty vector pcDNA3.1. A degree of colony formation inhibition similar to that of HBx was observed in 1762/1764 double mutation. Strikingly, 1753/1762/1764, 1762/1764/1768, or 1753/1762/1764/1768 mutations exhibited abrogation of the growth-suppressive effect (Figures 3A and 3B). The result indicated that BCP mutations affected the growth-suppressive effect of HBx in an accumulative manner.
Figure 3: Growth-suppressive effect of HBx mutants. A and B, Effects of HBx and artificial mutants on growth as assessed by the colony formation assay. HepG2 cells were transfected with plasmids expressing HBx constructed according to the naturally occurring mutation pattern of BCP/X or vector alone. C and D, Effects of HBx and artificial mutants on apoptosis as assessed by flow cytometry. Cells were stained with Annexin-V and PI V to determine cell viability and apoptosis, respectively. The percentage of cells that were Annexin-V positive, but PI negative were compared among different groups. Results are derived from triplicate transfections. *P<0.05; **P<0.01.
Annexin V apoptosis assay was performed to examine the apoptotic effects of HBx and artificial mutants. The early apoptotic and necrotic/ late apoptotic cells were depicted in Quadrant 4 and 1/2, respectively, in Figures 3C and 3D. While the effect of different HBx forms on apoptosis appeared obvious change in our system (Quadrant 4: HBx vs. control: 5.13–13.26% vs. 22.22%), HBx and artificial mutants decreased HepG2 cell death (Xwt, Xmt-2, Xmt-3-1, Xmt-3-2 and Xmt-4, 5.13, 6.58, 10.91, 8.45 and 13.26%, respectively) compared to the vector control (22.22%). Collectively, our data confirmed that the accumulative BCP mutations abrogated the growth-suppressive function of HBx.
The infection of HBV is a major factor contributing to the development of HCC in China and the mutation in HBx plays an important role in this process. However, the impact of BCP mutations on HBx activity was unclear. We characterized CP mutations based on the serum samples taken from patients infected with HBV/C1 from the Southern China (Guangdong province) with a higher prevalence of HCC. We showed that although the frequency of T1762/A1764 double mutation was high in HCC patients, but there was no difference compared with that of CH patients. In contrast, the adjacent V1753 or A1768 mutation significantly increased the risk of HCC. Recently, a longitudinal study demonstrated that T1762/A1764 mutation could be detected 4-7 years prior to HCC in most cases, while T1766/A1768 mutation occurred only at or near the stage of HCC, suggesting T1766/ A1768 may be a more valuable predictive marker for HCC . Because several reports demonstrated that T1766/A1768 mutation enhanced viral replication [29,32], high viral load caused by this mutation might contribute to hepatocarcinogenesis. The V1753 mutation had been reported was specifically associated with HBV C1 among Chinese HCC patients , particularly for HCC among HBeAg-positive-C1-carriers . Our study also confirmed that the V1753 mutation increases the risk of HCC in Chinese Guangdong with HBV/C1.
It is noteworthy that T1762/A1764 double mutation always accompanied with V1753 or A1768 point mutation. We found that the rates of V1753/T1762/A1764, T1762/A1764/A1768 or V1753/ T1762/A1764/A1768 mutations were significantly higher in HCC patients compared with that in CH patients. The accumulation of BCP mutations involving V1753 or/and A1768 in addition to T1762/A1764 were closely related to HCC among the patients infected with HBV/ C1, particularly for HBeAg-positive-carriers. Although T1762/A1764 mutation alone was not a triggering factors of HCC, T1762/A1764 with the additional V1753 or/and A1768 mutations in BCP region could be appreciably higher risk for hepatocarcinogenesis. Some in vitro study showed that the T1762/A1764 double mutation had no significant effect on replication capacity in comparison to the wild type . However, all CP mutants bearing additional mutations in this region, i.e., mutations at the position of V1753/T1762/A1764, T1762/A1764/ A1768 or V1753/T1762/A1764/A1768 exhibited to markedly enhance viral replication than the wild type [32,33], supporting a carcinogenetic potential of this mutations pattern. Thus, it is reasonable to think these mutations in BCP may play a synergistic role on enhancing HBV carcinogenesis.
Another feature of our study is that we try to understand the hepatocarcinogenesis potential of BCP mutations through HBV X protein (HBx) function. HBx is a multi-functional viral protein. It can modulate gene transcription, apoptosis, cell cycle, cell responses to genotoxic stress, protein degradation, and numerous signal transduction pathways  and, as a result, has been shown to induce cell transformation leading to HCC in a select strain of transgenic mice . Functional study revealed that the COOH-terminus of HBx plays a key role in regulating its transcriptional activity and cells proliferation . Since V1753, T1762, A1764 and A1768 mutations cause four amino acid (aa) substitutions changes at HBx COOH-terminus, i.e., 127(I-to-N/S/T), 130(K-to-M), 131(V-to-I) and 132(P-to-T), it is of interest to test whether BCP mutations affect the biological activity of HBx. We introduced a series of artificial mutants into a wild type HBx and constracted different HBx mutants. Transfection efficiency was monitored by comparing the relative amount of HBx by Western blot analysis. First, we analyzed the transactivation activity of different HBx through a luciferase assays. HepG2 cells were transiently co-transfected with NF-κB-Luc or mtNF-κB-Luc and HBx, and luciferase activity were examined. Like wild type HBx, T1762/A1764 double mutation induced a 4-5-fold increase in luciferase activity. This result indicated that T1762/A1764 double mutation alone did not impact the effect of HBx. However, the mutations at positions V1753/T1762/A1764, T1762/A1764/A1768 or V1753/T1762/A1764/A1768 largely inhibited the transactivation activity of HBx. In contrast, we found that none of HBx constructs could activate mtNF-κB-Luc. We suggest that the number of BCP variants may be involved in the transactivation function of HBx, and contributed to the development of HCC. However, the relationship between transcriptional activity and oncogenic potential of mutated HBx in hepatocarcinogenesis needs to be carefully analyzed in each mutation.
We next investigated the effects of different HBx on cells growth and apoptosis by a colony formation assay and flow cytometry. We noted that the trans-activation function of HBx is generally linked to its proapoptotic function. As expected, the expression of HBx caused a substantial reduction (5-fold) in the number of colonies compared with the control cells transfected with pcDNA3.1. A degree of colony formation inhibition similar to that of HBx was observed in T1762/ A1764 double mutation. Strikingly, V1753/T1762/A1764, T1762/ A1764/A1768 or V1753/T1762/A1764/A1768 mutations exhibited abrogation of the growth-suppressive effect. On the other hand, all HBx forms decreased HepG2 cells death compared to the vector control. However, the death rates of HepG2 expressing HBx and HBx mutant bearing T1762/A1764 double mutation were similar. While V1753/ T1762/A1764, T1762/A1764/A1768 or V1753/T1762/A1764/A1768 mutations have been shown to abrogate the apoptotic effects of HBx. These data further confirmed a synergistic role of BCP mutations could affect the function of HBx. However, the true impact of BCP mutations on the regulation of the biological function of HBx needs to be further evaluated.
We thank Dr. Yun Li for her technical help in luciferase assays. This study was supported by Guangdong Province Nature Science Foundation (9151040701000037) and Research Project of Guangzhou Science and Technology (2009J1-C321-2).