Journal of Oncology Research and Treatment
Open Access

Renal cell carcinomas (RCCs) are a heterogeneous group of tumors which account for around 90% of all adult renal malignancies [1]. The most common subtypes are clear cell (60-75%), followed by papillary (10-15%), chromophobe (5%) and collecting duct carcinoma [2]. Recent advances in the understanding of molecular alterations implicated in the pathogenesis of RCC have led to the development of a new subclassification of these tumors [3]. Translocation renal cell carcinoma (tRCC) is a newly recognized subtype of RCC with chromosomal translocations involving TFE3 (Xp11.2) or, less frequently, TFEB (6p21) [4]. Microphthalmia-associated transcription (MiT) family tRCC entails Xp11 tRCC and t (6;11) RCC. Xp11 tRCC and t (6;11) RCC are also known as TFE3- and TFEB-rearranged RCC, respectively. TFE3 and TFEB belong to the MiT family that regulates melanocyte and osteoclast differentiation. TFE3- and TFEB-rearranged RCC show distinguished clinicopathological and immunohistochemical features [5]. Xp11 tRCC comprises 20-40% of childhood RCC and about 4% of adult RCC with an average age of 50 years at diagnosis [6,7]. The natural history of Xp11 tRCC is variable from indolent behaviors [8,9] to aggressive disease that demonstrates lymph node and widespread metastasis. Xp11 tRCCs have the potential to metastasize as late as 20-30 years after diagnosis [7,10]. The associations between age, stage, grade of the disease, survival and recurrence of the disease have not been addressed in the literature. The aim of this study is to identify if these associations exist.

Study settings

The study was conducted at King Hussein Cancer Centre (KHCC), a comprehensive center that serves cancer patients in both inpatient and outpatient settings. RCC patients receive treatment according to KHCC-clinical practice guidelines (CPG). Approval was gained from the KHCC Institutional Review Board and the KHCC ethics committee. Data for all patients who underwent radical or partial nephrectomy and pathology that revealed tRCC between December 2005 and August 2017 were reviewed retrospectively.

Study design

All patients underwent staging assessment at the time of diagnosis, including clinical examination, blood tests, chest x-ray and computed tomography (CT) of the abdomen and pelvis. Pathological staging was performed using 2010 TNM classification system. Follow-ups were performed according to KHCC-CPGs, which included laboratory and radiological examinations according to the final TNM stage and tumor grade. Additional imaging was ordered as clinically indicated. For those with metastatic disease, tumors were evaluated by physical examination and CT scans at baseline and every 3 to 6 months. Overall disease responses were documented using RECIST criteria [11].

TFE3 stain was performed for tumors with histological features suggestive of tRCC, like papillary architecture and clear to eosinophilic cytoplasm. The diagnosis of Xp11 tRCC was confirmed by immunohistochemistry (IHC) analysis for nuclear TFE3 staining. Xp11.2 tRCC was analyzed by IHC staining to detect TFE3 in each tumor and tissue microarray block (catalog No. sc5958; Santa Cruz Biotechnology, Santa Cruz, CA, USA). Angiogenesis markers IHC analysis of the tumor tissue samples was performed by using the Ventana XT auto immunostainer (Roche, San Francisco, CA, USA) with the Optiview Dab Detection Kit (Roche) according to the manufacturer’s instructions. The results were independently evaluated by two specialized pathologists blind to the clinical data. The Fuhrman nuclear grading system, which uses a four point multi-parametric scale based on nuclear features, size, shape, color, and nucleolar prominence was used [12] as shown in Figures 1-3. Data including the patients’ characteristics, clinical manifestations, surgical techniques, pathological findings, radiology, and clinical outcomes was collected. Response to treatment based on cancer-specific survival (CCS), overall survival (OS) and progression-free survival (PFS) were analyzed. Patients were categorized into two group based on age group 1 (>40 years) and group 2 (≤ 40 years) and association between tumor grade, sex, disease free survival (DFS) and age were studied across groups.

Figure 1: The results were independently evaluated by two specialized pathologists blind to the clinical data.

Figure 2: The Fuhrman nuclear grading system, which uses a four point multi-parametric scale based on nuclear features, size, shape, color, and nucleolar prominence was used.

Figure 3: The Fuhrman nuclear grading system, which uses a four point multi-parametric scale based on nuclear features, size, shape, color, and nucleolar prominence was used.

Statistical analysis

The Chi-squared test was used to identify associations between results, responses, and demographics. The strength of associations was assessed by calculating odds ratios (ORs) and 95% confidence intervals. Relations between categorical and continuous variables were evaluated in independent t tests. Cross-tabulation was used to explore associations between categorical data, and chi-squared tests were used to assess the statistical significance of associations. P values less than 0.05 were considered statistically significant.

Clinical characteristics

Twenty-three patients were identified, 15 (65%) of the patients were males. The mean age at diagnosis was 37 years. The majority of the patients (65%) were diagnosed incidentally during abdominal imaging for other indications. The remaining 35% suffered from pain, gross hematuria, metastasis, abdominal distension or weight loss at the time of the first presentation. Patients’ characteristics are shown in Table 1.

Table 1: Demographic data of patient population.

The tumor was on the right kidney in 52.2% of the patients, and bilateral in one patient. Ten patients (43.5%) were above 40 years of age, and 13 patients (56.5%) were less than or equal to 40 years old. Regarding the tumor characteristics, the mean tumor size was 9 cm; 4 patients (17.3%) had pathologic T1, seven patients (30.4%) had pathologic T2, 10 patients (43.4%) had pathologic T3 or more and two patients (8.7%) had no surgery and thus were not evaluated for pathologic T staging. Eleven patients (47.8%) had lymph node dissection for clinically enlarged lymph nodes and seven (64%) of them had lymph nodes metastasis. Patients were categorized into two group based on age group 1 (Table 2.

Table 2: Disease-free period and site of recurrence and metastasis.

Surgical intervention

Nineteen patients (83%) underwent radical nephrectomy and two (8%) had partial nephrectomy. Bilateral renal tumors were managed with left radical and right partial nephrectomy. Eighteen patients (78%) had an open approach and three patients (13%) had a laparoscopic approach. Eight patients (35%) had metastasis, and three of the eight patients had metastasis at the time of diagnosis. Six patients (26%) received sunitinib for their metastatic disease, and one of them had a complete response with a disease free period of 18 months. Four patients had a stable disease for an average of 19 months, and only one had disease progression without any response (Table 3). There were no differences in DFS in patients above and below 40 years of age. No statistically significant associations between disease-free period postoperatively, sex, pathologic T staging, overall survival and age were found across the groups, using 40 years as a cut point (Figures 4 and 5).

Table 3: T staging of patients (n=23).

Figure 4: No statistically significant associations between Disease-Free Period postoperatively, sex, pathologic T staging, overall survival and age were found across the groups, using 40 years as a cut point.

Figure 5: No statistically significant associations between Disease-Free Period postoperatively, sex, pathologic T staging, overall survival and age were found across the groups, using 40 years as a cut point.

Three patients died during follow-up time; one patient died 4 months after surgery, one 21 months after surgery (both due to metastasis) and one died of brain hemorrhage. The mean follow-up time is 35 months, and 3-year disease overall free survival was 75%.

The median age at diagnosis of RCC is approximately 64, according to the 2003 to 2007 National Cancer Institute (NCI) and the Surveillance, Epidemiology and End Results (SEER) Cancer Statistics Review. RCC is unusual in patients under 40 years of age and is rare in children [13- 15]. tRCC, is a distinct variant of RCC and is associated with fusion of the TFE3 gene to other genes located on chromosome Xp11.2 [8,9,16]. Xp tRCC was initially thought to be a malignancy of pediatrics and young adults. However, more recent data has increasingly identified this tumor in older adults as well [17]. Adequate immunohistochemical workup is not routinely done for adults with RCC, which results in most cases of adult tRCC misdiagnosed as conventional [18].

Furthermore, the similar gross and histological findings to other RCCs also contributed to the underestimation of tRCC frequency in adults [19].

Translocation carcinoma tens to occur at a younger age compared with other RCCs: In our study the mean age at diagnosis is 37 years and is more common in males (65%) than in females.

Nevertheless, current data depending on many cohort studies as well as some meta-analyses show that the course of tRCC is more indolent in pediatric populations. Adults tend to present with advanced stage and distant metastases and thus have a poorer prognosis [17,20].

The treatment of Xp11.2 tRCC remains a major challenge for health care providers. Many advances in the treatment of clinically localized and metastatic RCC disease have been accomplished [21]. This is not the case in tRCC management due to the small number of patients to run clinical trials. Thirty cases of t(6;11) RCC were reported in the International Society of Urological Pathology Conference. Of these, the majority of pediatric cases were indolent with recurrence in only 17% of them. Most of the adult population in the same study presented with metastasis or pursued an aggressive clinical course causing death [22]. Another study which focused on regional lymph node metastasis as a prognostic factor for tRCC showed that regional lymph node metastasis in pediatrics doesn’t necessarily predict poor prognosis as it nearly does in adults [8]. Another multivariate analysis by Peckova et al. demonstrated that the more aggressive t(6,11) RCC appeared to affect older patients (mean age of 45.2 years) rather than younger patients (mean age of 31.5 years) [23]. The management of clinically localized Xp11.2 tRCC is similar to general RCC guidelines [24]. Currently, there is no standard treatment for patients with MiT family tRCC. Most studies depend on the PFS, CSS and OS as indicators of the efficiency of different treatment modalities. Currently, the most favorable outcomes have been reported with patients who undergo a radical nephrectomy and lymph node dissection before the development of metastasis. However, the true challenge lies in the postoperative follow-up process; special follow-up protocols are seemingly promising in improving the quality of life of tRCC adult patients and in prolongation of the three mentioned parameters [25]. Future studies will focus on genomic technologies to identify prognostic factors and therapeutic targets in tRCC. One study found that high chromosome copy number alterations in Xp11 tRCC are associated with aggressive behavior [24]. Another found that chromosome 17q gain correlated with older age and worse outcome which corroborates the effect of age reported by Ellis et al. [10]. The scope of this study is to address the association between age (> or ≤ 40 years) and disease free period postoperatively, sex, pathologic T staging, and overall survival in patients with tRCC treated in KHCC. No significant associations were found across the groups.

This study is limited by a retrospective design and the absence of long-term follow-up. Furthermore, the small sample size makes it difficult to draw a definitive conclusion regarding associations between age and the factors studied.

tRCC, is a variant of RCC with unique characteristics. tRCC has variable clinical behavior from indolent to aggressive disease. Age may not affect this clinical behavior. Future studies with larger sample size and a longer follow up period is required.