Nanobiosensors: Next Generation Point-of-Care Biomedical Devices for Personalized Diagnosis
Kuldeep Mahato1, Alisha Prasad1, Pawan Kumar Maurya2 and Pranjal Chandra1*
1Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati-781 039, Assam, India
2Interdisciplinary Laboratory for Clinical Neuroscience (LiNC), Department of Psychiatry, Universidade Federal de Sao Paulo- UNIFESP, Sao Paulo, Brazil
- *Corresponding Author:
- Pranjal Chandra
Department of Biosciences and Bioengineering
Indian Institute of Technology
Guwahati-781 039, Assam, India
E-mail: [email protected]
Received Date: February 26, 2016 Accepted Date: February 28, 2016 Published Date: March 04, 2016
Citation: Mahato K, Prasad A, Maurya PK, Chandra P (2016) Nanobiosensors: Next Generation Point-of-Care Biomedical Devices for Personalized Diagnosis. J Anal Bioanal Tech 7:e125. doi:10.4172/2155-9872.1000e125
Copyright: © 2016 Mahato K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Imagine an era where patients will be able to tell the doctor that they themselves have detected the target molecule (e.g., antigen, proteins, chemical molecule, etc.) related to a disease using a personalized diagnostic tools (e.g., glucose for diabetes, Salmonella typhi for typhoid, bilirubin for anemia). This may help the clinicians to quickly design the adequate therapeutic strategies for patients. A biosensor based diagnostic technology promises to do just that-quick onsite diagnosis. Biosensors in general are stand alone miniature portable devices that can detect range of analytes quickly with the help of transducers that converts the biological response into measurable analytical signals. It represents a rapidly expanding field currently and is expected to reach $22.68 billion market by 2020. Moving this proof-of-concept device for everyday analysis of biological or chemical samples has involved tremendous development in biosensors research . Therefore, many such diagnostic devices are available in the global market for various medical purpose such as; malaria testing kit [(CareStart™ Malaria HRP2/PLDH; Access Bio, United States) (Maleriscan® Malaria Pf/ PAN; Bhat Bio-Tech (P) Ltd., India)], hCG based pregnancy testing kits [(Pregnosis®; Hoffmann-La Roche Ltd., Montreal), (Velocit™; Dr.Reddy’s, India)], and portable glucometers [(CareStart™ G6PD, Access Bio, United States)] etc. Although these miniaturized devices are extensively fabricated and possess good market potential, research to improvise the existing designs in terms of relativity, sensitivity, detection time, and point-of-care diagnosis are still underway .
Research and development to design biosensor prototypes has been rapid and ever expanding. To overcome the challenges related to sensitivity in detecting and capturing target molecules, introduction of “nanotechnology” has been found to be extensively useful. The advances in nanotechnology offers sensitive and specific detection of analyte by: (i) integrating new novel microfluidic/nanofluidics technologies to design small, and easy-to-use devices , (ii) surface functionalization of biomaterials on nanocomposites, and (iii) new signal amplification strategies using various nanomaterials [4,5]. The developments in nanotechnology and changes in electronic, thermal, and optical property of nanoscale materials have brought new detection methods in biosensing [6-8]. Research and development in the field of nanobiosensors is wide and multidisciplinary, spanning knowledge of researchers from diverse backgrounds such as biological science, medical science, material science, physics, chemistry, electronics, mechanical engineering, and other engineering sciences to develop various types of nanobiosensor prototypes [7,9-11]. Tremendous work has been done on next generation fabrication of , semisynthetic  and synthetic ligands , plastic antibodies , catalytic electrodes , bioinspired nanotechnologies for sensing, therapy, and controlled surface for cellular interaction , in vivo  and in vitro [19,20] devices for regenerative medicine, arrays, biomimetics and tele-interfaces for digital health . These platforms may help to design commercially viable nanobiosensor for various biomedical applications. Among the available nanobiosensor types, electrochemical readout system is considered to be most powerful due to its high sensitivity and ability to be miniaturized for fast bimolecular analysis , thereby helping in designing therapeutic strategies quickly to overcome patients stress [20,23].
Researchers have developed a highly sensitive and interferencefree endotoxin detection system using new nanobiomaterials and nanobioconjugated paper/plastic settings. Prototypes for immunosensors , lateral flow based biosensors , microfluidic sensor system , enzyme sensor , paper inkjet sensor  etc. for detection of various clinically important target molecules including parasite, hormones, cancer biomarkers, heavy metal ions, cancer cells, and bacteria have been developed. In current years, aptamers has been extensively studied in diagnostics and therapeutics to design novel nanobiosensors. Aptamers have been used to design singledisposable nanobiosensors that can integrate multiple biophysical and biochemical processes even at low concentrations with high sensitivity. In a recent report a "real-time aptamer based biosensor" has been developed  that continuously measure specific biomolecules directly in living animals. Recent reports demonstrates the ability of aptamers to diagnose pancreatic ductal adenocarcinoma for in vivo imaging and clinical tissue recognition  and detection of human liver cancer cells . Electrochemically tuned nanostructures has also been applied to design new nanobiosensor prototypes [31,32]. Researchers have reported tuned molecular probe / surface for ultrasensitive biomolecular detection of virus , circulating nucleic acids , and circulating cancer cells .
Currently smartphones because of its handy, well equipped, and classy features such as: advanced processors, increased memory, high-resolution camera, high-end security via fingerprinting, and a variety of built-in sensors has been used to design nanobiosensor prototypes. Based on the idea to design personalised nanobiosensor settings, extensive work has been done on portable detection system using high resolution smartphone cameras . Notable work using this technology has been done in in-vitro and real-time monitoring of clinical condition. The data produced by smartphone based in vitro diagnostics can be added with spatial and temporal information, which can be used for monitoring and management in critical situations . Research on smartphone based nanobiosensor prototypes such as: lateral flow assays (LFA), microscopy, electrochemical sensing, immunoassays, surface plasmon resonance-based biosensing, flow cytometry, and optical detection has been reported . Examples of few commercialized smartphone based prototypes are iHealth, AliveCor, GENTAG, Mobile Assay, and CellScope etc.
Though the reports mentioned above clearly show tremendous promise in nanobiosensor research and its continuous development, still there is incredible scope to design new sensing strategies and improve the known sensing strategies. The future work should be directed towards the development of new, sensitive, selective, quick, and personalized diagnosis strategies for bimolecular analysis.
Dr. Pranjal Chandra thanks to Indian Institute of Technology, Guwahati, India for providing necessary facilities to prepare this editorial.
- Chandra P (2013) Miniaturized Multiplex Electrochemical Biosensor in Clinical Bioanalysis. J Bioanal Biomed 5: e122.
- Gubala V, Harris LF, Ricco AJ, Tan MX, Williams DE (2012) Point of care diagnostics: status and future. Anal Chem 84: 487-515.
- Noh HB, Chandra P, Kim YJ, Shim YB (2012) A simple separation method with a microfluidic channel based on alternating current potential modulation. Anal Chem 84: 9738-9744.
- Chandra P, Singh J, Singh A, Srivastava A, Goyal RN, et al. (2013) Gold nanoparticles and nanocomposites in clinical diagnostics using electrochemical methods. Journal of Nanoparticles 535901.
- Srivastava A, Srivastava A, Srivastava A, Chandra P (2015) Marine biomaterials in therapeutics and diagnostic. In: Springer Handbook of Marine Biotechnology. Springer, pp: 1247-1263.
- Zhu Y, Chandra P, Song KM, Ban C, Shim YB (2012) Label-free detection of kanamycin based on the aptamer-functionalized conducting polymer/gold nanocomposite. Biosens Bioelectron 36: 29-34.
- Chandra P, Noh HB, Won MS, Shim YB (2011) Detection of daunomycin using phosphatidylserine and aptamer co-immobilized on Au nanoparticles deposited conducting polymer. Biosens Bioelectron 26: 4442-4449.
- Chandra P, Noh HB, Shim YB (2013) Cancer cell detection based on the interaction between an anticancer drug and cell membrane components. Chem Commun (Camb) 49: 1900-1902.
- Chandra P, Zaidi SA, Noh HB, Shim YB (2011) Separation and simultaneous detection of anticancer drugs in a microfluidic device with an amperometric biosensor. Biosens Bioelectron 28: 326-332.
- Chandra P, Son NX, Noh HB, Goyal RN, Shim YB (2013) Investigation on the downregulation of dopamine by acetaminophen administration based on their simultaneous determination in urine. Biosens Bioelectron 39: 139-144.
- Chandra P, Das D, Abdelwahab AA (2010) Gold Nanoparticles in Molecular Diagnostics And Therapeutics. Digest Journal of Nanomaterials & Biostructures 5: 363-367.
- Parlak O, Ashaduzzaman M, Kollipara SB, Tiwari A, Turner AP (2015) Switchable Bioelectrocatalysis Controlled by Dual Stimuli-Responsive Polymeric Interface. ACS applied materials & interfaces 7: 23837-23847.
- Azzouzi S, Patra HK, Ali MB, Abbas MN, Dridi C, et al. (2016) Citrate-selective electrochemical µ-sensor for early stage detection of prostate cancer. Sensors and Actuators B: Chemical 228: 335-346.
- Kashefi-Kheyrabadi L, Mehrgardi MA, Wiechec E, Turner AP, Tiwari A (2014) Ultrasensitive detection of human liver hepatocellular carcinoma cells using a label-free aptasensor. Anal Chem 86: 4956-4960.
- Chandra P, Noh HB, Pallela R, Shim YB (2015) Ultrasensitive detection of drug resistant cancer cells in biological matrixes using an amperometric nanobiosensor. Biosens Bioelectron 70: 418-425.
- Noh HB, Lee KS, Chandra P, Won MS, Shim YB, et al. (2012) Application of a Cu–Co alloy dendrite on glucose and hydrogen peroxide sensors. Electrochimica Acta 61: 36-43.
- Blind M, Blank M (2015) Aptamer selection technology and recent advances. Molecular Therapy-Nucleic-Acids 4: e223.
- Noh HB, Chandra P, Moon JO, Shim YB (2012) In vivo detection of glutathione disulfide and oxidative stress monitoring using a biosensor. Biomaterials 33: 2600-2607.
- Chandra P, Koh WC, Noh HB, Shim YB (2012) In vitro monitoring of i-NOS concentrations with an immunosensor: the inhibitory effect of endocrine disruptors on i-NOS release. Biosens Bioelectron 32: 278-282.
- Yadav SK, Agrawal B, Chandra P, Goyal RN (2014) In vitro chloramphenicol detection in a Haemophilus influenza model using an aptamer-polymer based electrochemical biosensor. Biosensors and Bioelectronics 55: 337-342.
- Turner AP (2013) Biosensors: sense and sensibility. Chem Soc Rev 42: 3184-3196.
- Zhu Y, Chandra P, Shim YB (2013) Ultrasensitive and selective electrochemical diagnosis of breast cancer based on a hydrazine-Au nanoparticle-aptamer bioconjugate. Anal Chem 85: 1058-1064.
- Kurbanoglu S, Mayorga-Martinez CC, Medina-Sánchez M, Rivas L, Ozkan SA, et al. (2015) Antithyroid drug detection using an enzyme cascade blocking in a nanoparticle-based lab-on-a-chip system. Biosens Bioelectron 67: 670-676.
- Mars A, Parolo C, de la Escosura-Muñiz A, Raouafi N, Merkoçi A (2016) Control of Electron-transfer in Immunonanosensors by Using Polyclonal and Monoclonal Antibodies. Electroanalysis 28: 1-9.
- Chamorro-Garcia A, Dela Escosura-Muñiz A, Espinosa-Castañeda M, Rodriguez-Hernandez CJ, de Torres C, et al. (2016) Detection of parathyroid hormone-like hormone in cancer cell cultures by gold nanoparticle-based lateral flow immunoassays. Nanomedicine 12: 53-61.
- de la Escosura-Muñiz A, Merkoçi A (2015) Nanochannels for electrical biosensing. TrAC Trends in Analytical Chemistry.
- da Silva ET, Miserere S, Kubota LT, Merkoçi A (2014) Simple on-plastic/paper inkjet-printed solid-state Ag/AgCl pseudoreference electrode. Anal Chem 86: 10531-10534.
- Ferguson BS, Hoggarth DA, Maliniak D, Ploense K, White RJ, et al. (2013) Real-time, aptamer-based tracking of circulating therapeutic agents in living animals. Science translational medicine 5: 213ra165-213ra165.
- Wu X, Zhao Z, Bai H, Fu T, Yang C, et al. (2015) DNA Aptamer Selected against Pancreatic Ductal Adenocarcinoma for in vivo Imaging and Clinical Tissue Recognition. Theranostics 5: 985-994.
- Xu J, Teng IT, Zhang L, Delgado S, Champanhac C, et al. (2015) Molecular Recognition of Human Liver Cancer Cells Using DNA Aptamers Generated via Cell-SELEX. PLoS One 10: e0125863.
- Sekretaryova AN, Eriksson M, Turner AP (2015) Bioelectrocatalytic systems for health applications. Biotechnol Adv S 0734-9750: 30060-30064.
- Oh SS, Lee BF, Leibfarth FA, Eisenstein M, Robb MJ, et al. (2014) Synthetic aptamer-polymer hybrid constructs for programmed drug delivery into specific target cells. J Am Chem Soc 136: 15010-15015.
- Rackus DG, Dryden MD, Lamanna J, Zaragoza A, Lam B, et al. (2015) A digital microfluidic device with integrated nanostructured microelectrodes for electrochemical immunoassays. Lab Chip 15: 3776-3784.
- Green BJ, Saberi Safaei T, Mepham A, Labib M, Mohamadi RM, et al. (2016) Beyond the Capture of Circulating Tumor Cells: Next-Generation Devices and Materials. Angew Chem Int Ed Engl 55: 1252-1265.
- Walzik MP, Vollmar V, Lachnit T, Dietz H, Haug S, et al. (2015) A portable low-cost long-term live-cell imaging platform for biomedical research and education. Biosens Bioelectron 64: 639-649.
- Park YM, Han YD, Kim KR, Zhang C, Yoon HC et al. (2015) An immunoblot-based optical biosensor for screening of osteoarthritis using a smartphone-embedded illuminometer. Analytical Methods 7: 6437-6442.
- Roda A, Michelini E, Zangheri M, Fusco MD, Calabria D, et al. (2015) Smartphone-based biosensors: A critical review and perspectives. TrAC Trends in Analytical Chemistry.