alexa Accumulation of the Mutations in Basal Core Promoter of Hepatitis B Virus Subgenotype C1 Increase the Risk of Hepatocellular Carcinoma in Southern China| Abstract
ISSN: 2161-0681

Journal of Clinical & Experimental Pathology
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  • Research Article   
  • J Clin Exp Pathol 2013, Vol 3(2): 141
  • DOI: 10.4172/2161-0681.1000141

Accumulation of the Mutations in Basal Core Promoter of Hepatitis B Virus Subgenotype C1 Increase the Risk of Hepatocellular Carcinoma in Southern China

Weihua Li1*, Guangyuan Chen1, Xianwen Yu1, Yongying Shi1, Miaoguan Peng1 and Jianjun Wei2
1Department of Infectious Disease, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou, Guangdong Province, China
2Hepatitis Research Room, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510102, Guangdong Province, China
*Corresponding Author : Weihua Li, Department of Infectious Disease, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou, Guangdong Province, China, Tel: 86-20-83062268, Fax: 86-20-83062245, Email: [email protected]

Received Date: May 08, 2013 / Accepted Date: Jun 12, 2013 / Published Date: Jun 14, 2013

Abstract

Background: Hepatitis B virus (HBV) genotype C is associated with the development of Hepato Cellular Carcinoma (HCC). In addition, HBV subgenotype C1 is the major subgenotype in Southern China. The aim of this study was to investigate whether there was the specific mutation patterns in HBV/C1 associated with Southern Chinese patients with HCC.

Methods: Mutations in HBV Basal Core Promoter (BCP) and their association with HCC were assessed in a matched cross-sectional control study of 102 HCC and 105 Chronic Hepatitis (CH) patients (from Guangdong, China) infected with HBV/C1. Functional analysis of HBx mutants was performed by the colony formation assay and the luciferase assays.

Results: T1762/A1764 double mutations was frequently found in patients infected with HBV/C1, regardless of clinical status (64.7% in HCC and 51.4% in CH, P>0.05). Unexpectedly, the adjacent V1753 or A1768 mutation significantly increased the risk of HCC (P<0.05). Moreover, the prevalence of triple or quadruple mutations in BCP was significantly higher in patients with HCC than those with CH, particularly for HBeAg-positive-carriers (P<0.05). Functional analysis revealed that T1762/A1764 mutation alone did not alter the transcriptional activity and the inhibitory effects on cell proliferation and apoptosis of HBx, but triple or quadruple mutations largely abrogated this effect.

Conclusions: Accumulation of mutations involving V1753 or/and A1768 in addition to T1762/A1764 in BCP region were closely related to HCC among the patients infected with HBV/C1, particularly for HBeAg-positive-carriers. The increased risk of HCC caused by BCP variants may be attributable partially to modifying the biological functions of HBx.

Keywords: Hepatitis B virus; Basal core promoter; X gene; Mutation; Hepatocellular carcinoma

Citation: Li W, Chen G, Yu X, Shi Y, Peng M, et al. (2013) Accumulation of the Mutations in Basal Core Promoter of Hepatitis B Virus Subgenotype C1 Increase the Risk of Hepatocellular Carcinoma in Southern China. J Clin Exp Pathol 3:141. Doi: 10.4172/2161-0681.1000141

Copyright: © 2013 Li W, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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