Abstract

The plant world is a rich source of bioactive substances, many of which have been utilized therapeutically to treat a variety of diseases since prehistory. These metabolites have gained recent attention for their ability to treat a variety of malignancies using multiple pathways. Some of these substances include alkaloids or glycosides, such as podophyllotoxin (PPT), anaryltetralin lignan, and others that have shown promise as anti-cancer medications.Alkaloids come in three main types: quinoline alkaloids, diterpenoid alkaloids, and indole alkaloids. In this study, numerous plant biotechnology techniques that are employed to increase the production of these anticancer compounds in various species are discussed. Numerous in vitro culture methods, including hairy roots and stimulation of suspension culture, are useful in this area. In this context, a variety of in vitro culture techniques are being employed to study the effects of plant growth regulators and elicitors on different explants, including stimulation of suspension culture and hairy roots.