alexa

GET THE APP

Bicyclic Eremophilane-type Petasite Sesquiterpenes Potentiate Peroxisome Proliferator-activated Receptorand#160; and#947; Activator-mediated Inhibition of Dendritic Cells| Abstract

Journal of Molecular Immunology
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
  • Research Article   
  • J Mol Immunol 2018, Vol 3(1): 115

Bicyclic Eremophilane-type Petasite Sesquiterpenes Potentiate Peroxisome Proliferator-activated Receptor  γ Activator-mediated Inhibition of Dendritic Cells

Narcy Arizmendi1, Chenjie Hou2, Fujiang Guo2, Yiming Li2 and Marianna Kulka1*
1Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta, T6G 2E1, Canada
2School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China
*Corresponding Author : Marianna Kulka, National Institute for Nanotechnology, National Research Council Canada, 11421 Saskatchewan Drive, Edmonton, AB, T6G 2M9, Canada, Tel: 780-641-1687, Email: [email protected]

Received Date: Sep 21, 2017 / Accepted Date: Nov 20, 2017 / Published Date: Nov 27, 2017

Abstract

Background: Dendritic cell (DC) activation induces expression of co-stimulatory surface molecules, as well as migration into secondary lymphoid organs, where they activate naïve T cells. A family of plant derivatives, eremophilanetype petasite sesquiterpenes can regulate the immune system through DC targeting due to their anti-inflammatory effects. Peroxisome proliferator activated receptor gamma (PPARγ) is involved in inhibition of inflammatory responses and induction of DCs to acquire a mucosal phenotype.
Objective: Since mucosal DCs are central in innate immune responses, we hypothesized that eremophilane-type petasite sesquiterpenes exerted their anti-inflammatory effects by inhibiting DC maturation and activation, through PPARγ.
Methods: This study assessed the bicyclic eremophilane-type petasite sesquiterpene compounds Fukinone and 10βH-8α,12-Epidioxyeremophil-7(11)-en-8β-ol (ZYFDC21 and ZYFDC22), in the maturation and activation of mouse dendritic cells, measured by surface expression of co-stimulatory molecules and cytokine production upon LPS stimulation, in the presence or absence of PPARγ agonists. DCs were generated from mouse bone marrow cells in media supplemented with GM-CSF+IL-4, and were harvested on day 8 and activated for 3 h with bicyclic eremophilanetype petasite sesquiterpenes ZYFDC21 or ZYFDC22 in presence or absence of synthetic PPARγ agonists (GW1929, TGZ), or the natural PPARγ ligand 15d-PGJ2, followed by overnight activation with LPS.
Results: Effects on DC maturation were evaluated by surface expression of the co-stimulatory molecule CD86 by flow cytometry, and for DC activation, by relevant cytokines released in cell-free supernatants measured by ELISAs. We observed differences in the upregulation of surface expression of CD86, along with release levels of TNF, IL-6 and IL-12p70 in DCs stimulated with LPS when using combinations of bicyclic eremophilane-type petasite sesquiterpenes ZYFDC21 or ZYFDC22, and PPARγ agonists, in particular the PPARγ ligand 15d-PGJ2.
Conclusion: These results indicate that bicyclic eremophilane-type petasite sesquiterpenes Fukinone and 10βH- 8α,12-Epidioxyeremophil-7(11)-en-8β-ol inhibit maturation and activation of DC, and this activity is augmented upon PPARγ activation.

Keywords: Inflammation; Plant derivatives; Transcription factor; Peroxisome proliferator activated receptor gamma

Citation: Arizmendi N, Hou C, Guo F, Li Y, Kulka M (2018) Bicyclic Eremophilane-type Petasite Sesquiterpenes Potentiate Peroxisome Proliferator-activated Receptor γ Activator-mediated Inhibition of Dendritic Cells. J Mol Immunol 3: 115.

Copyright: © 2017 Arizmendi N, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Select your language of interest to view the total content in your interested language

Post Your Comment Citation
Share This Article
Article Usage
  • Total views: 1916
  • [From(publication date): 0-2018 - Sep 19, 2021]
  • Breakdown by view type
  • HTML page views: 1713
  • PDF downloads: 203
Top