alexa B-lymphocyte, Macrophage and Mast Cell Density in the Stroma Underlying HPV-Related Cervical Squamous Epithelial Lesions and their Relationship to Disease Severity: an Immunohistochemical Study| Abstract
ISSN: 2161-0681

Journal of Clinical & Experimental Pathology
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
  • Research Article   
  • J Clinic Experiment Pathol 2012, Vol 2(2): 105
  • DOI: 10.4172/2161-0681.1000105

B-lymphocyte, Macrophage and Mast Cell Density in the Stroma Underlying HPV-Related Cervical Squamous Epithelial Lesions and their Relationship to Disease Severity: an Immunohistochemical Study

Periklis G. Foukas1#, Argyroula P. Zourla1#, Sotirios Tsiodras2*#, Athanasios Tsanas3, Konstantinos Leventakos1,2, Ekaterini Chranioti4, Aris Spathis4, Christos Meristoudis4, Charalambos Chrelias5, Dimitrios Kassanos5, Georgios Petrikkos2, Petros Karakitsos4 and Ioannis G. Panayiotides1
12nd Department of Pathology, University of Athens Medical School, Attikon University Hospital, Greece
24th Department of Internal Medicine, University of Athens Medical School, Attikon University Hospital, Greece
3Systems Analysis Modelling and Prediction Group, Oxford Centre for Industrial and Applied Mathematics, University of Oxford, UK
4Department of Cytopathology, University of Athens Medical School, Attikon University Hospital, Greece
53rd Department of Obstetrics and Gynecology, University of Athens Medical School, Attikon University Hospital, Greece
#Contributed equally to this work
*Corresponding Author : Sotirios Tsiodras MD, PhD, 4th Department of Internal Medicine, University of Athens Medical School, Attikon University Hospital), 1 Rimini street, GR-12462 Haidari, Athens, Greece, Tel: 30-210-5831950, Fax: 30-210-5831949, Email: [email protected]

Received Date: Dec 06, 2011 / Accepted Date: Jan 28, 2012 / Published Date: Feb 01, 2012

Abstract

Introduction: B lymphocyte, macrophage and mast cell densities in the stroma underlying cervical low and high grade squamous intraepithelial lesions (LSIL and HSIL) and squamous cell carcinoma (SCC) in 200 tissue samples, with concomitant HPV typing, was assessed, in order to examine their relationship to disease status and progression.

Methods: Sections from 215 cervical specimens (149 LSIL, 38 HSIL, 13 SCC and 15 normal cervical mucosae) were immunostained for B lymphocytes (CD20), macrophages (CD68) and mast cells (CD117). The number of cells per high power field (henceforth called density) in the stroma underlying epithelial lesions was assessed. Statistical analysis was performed using four ordinal scale groups of increasing severity (normal, LSIL, HSIL and SCC).

Results: Densities of all three cell types had a statistically significant, proportional correlation to disease severity, more so for B lymphocytes. Increased density of any cell type is linked to an increase in the densities of the remaining two. A statistically significant difference in B lymphocyte and mast cell density was found between LSIL and HSIL. No cell type density was found to be predictive of the outcome of LSIL. No relationship with HPV type was found.

Discussion:Increased B lymphocyte and mast cell density in the stroma underlying cervical HSIL and SCC compared to LSIL suggests a possible relationship of both cell types to progression of cervical SIL. No predictive value of the density of any cell type was found concerning the outcome of LSIL. Immunohistochemistry may contribute to elucidate the relationship of local immunity effector cells to cervical epithelial lesions.

Keywords: HPV; SIL; B-lymphocyte; Mast cell; Macrophage

Citation: Foukas PG, Zourla AP, Tsiodras S, Tsanas A, Leventakos K et al. (2012) B-lymphocyte, Macrophage and Mast Cell Density in the Stroma Underlying HPVRelated Cervical Squamous Epithelial Lesions and their Relationship to Disease Severity: an Immunohistochemical Study. J Clinic Experiment Pathol 2:105. Doi: 10.4172/2161-0681.1000105

Copyright: © 2012 Foukas PG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Select your language of interest to view the total content in your interested language

Post Your Comment Citation
Share This Article
Article Usage
  • Total views: 12551
  • [From(publication date): 3-2012 - May 21, 2019]
  • Breakdown by view type
  • HTML page views: 8725
  • PDF downloads: 3826
Leave Your Message 24x7
Top