ISSN: 2155-6199

Journal of Bioremediation & Biodegradation
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
  • Research Article   
  • J Bioremediat Biodegrad 9:421,
  • DOI: 10.4172/2155-6199.1000421

Carbonic Anhydrase II Based Biosensing of Carbon Dioxide at High Temperature: An Analytical and MD Simulation Study

Danish Idrees1*, Razique Anwer2, Mohd Shahbaaz3, Myalowenkosi Sabela4, Khalid I Al Qumaizi5, Osama A Al Khamees6, Samudrala Gourinath1, Manoj Kumar7 and MP Singh7
1School of Life Sciences, Jawaharlal Nehru University, Delhi, India
2Department of Anatomy (Microbiology), College of Medicine, Al Imam Mohammad Ibn Saud Islamic University (IMSIU), , Riyadh, Saudi Arabia
3South African National Bioinformatics Institute, University of the Western Cape, Private BagX17, Bellville, Cape Town, South Africa
4Department of Chemistry, Durban University of Technology, Durban 4000, South Africa
5Department of Family Medicine, College of Medicine, Al Imam Mohammad Ibn Saud Islamic University (IMSIU), , Riyadh, Saudi Arabia
6Department of Pharmacology, College of Medicine, Al Imam Mohammad Ibn Saud Islamic University (IMSIU), , Riyadh, Saudi Arabia
7Indian Oil Corp Ltd, Research and Development Centre, , Faridabad, India
*Corresponding Author : Danish Idrees, School of Life Sciences, Jawaharlal Nehru University, Delhi, India, Tel: 9911651302, Email: danish.idress@gmail.com

Received Date: Nov 14, 2017 / Accepted Date: Nov 23, 2017 / Published Date: Nov 27, 2017

Abstract

Concentration of carbon dioxide (CO2) in the atmosphere has increased significantly due to anthropogenic activities and attributed as a major factor to global warming. Its detection by biosensing methods will provide an alternative for the assessment of CO2 concentration. Biomineralization of CO2 is one of the available methods for the biological conversion of CO2 to carbonate using a highly active enzyme, carbonic anhydrase II (CAII). CAII was used for the carbonation reaction to convert CO2 to CaCO3. The precipitation of calcium carbonate (CaCO3) was promoted in the presence of the CAII at 325 K. CAII showed an enhanced formation of solid CaCO3 through the acceleration of CO2 hydration rate at 325 K. Furthermore, the electrocatalytic properties of glassy carbon electrode enable us to determine the reduction peak potential values of CO2 through cyclic voltammetry at –1.75 and 0.3 V at 325 K. Molecular dynamic (MD) simulations were performed each at 50 ns time scale provided a deeper insight into the molecular basis of the CAII interaction with CO2 at different temperatures, highlighted that the CAII can detect CO2 up to 325 K. We assume that CAII could be an effective and economical biosensor for biomineralization of CO2 at high temperature 325 K.

Keywords: Carbon dioxide sequestration; Calcium carbonate; Biomineralization; MD simulations; Carbonic anhydrase II

Citation: Idrees D, Anwer R, Shahbaaz M, Sabela M, Al Qumaizi KI, et al. (2018) Carbonic Anhydrase II Based Biosensing of Carbon Dioxide at High Temperature: An Analytical and MD Simulation Study. J Bioremediat Biodegrad 9: 421. Doi: 10.4172/2155-6199.1000421

Copyright: © 2018 Idrees D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Top