alexa Comparative Quantitation of Lymphoma Gene Signatures in Parallel Formalin Fixed Paraffin Embedded and Frozen Tissue Lymph Nodes
ISSN: 2161-0681

Journal of Clinical & Experimental Pathology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Comparative Quantitation of Lymphoma Gene Signatures in Parallel Formalin Fixed Paraffin Embedded and Frozen Tissue Lymph Nodes

Ebrahim Sakhinia1,2*, Jordan Goodchild3, Lia Patricia Menasce4, Joan Radford5, Mehrdad Asghari Estiar1, Masoud Sakhinia6 and Richard Byers3

1Department of Medical Genetic, Faculty of Medicine, Tabriz Molecular Analysis Centre (TGAC) and Tuberculosis and Lung Diseases research center, Tabriz university of Medical Science, Tabriz, Iran

2Division of Regenerative Medicine, School of Medicine, Faculty of Medical and Human Sciences, the University of Manchester, UK

3Department of Histopathology, Manchester Royal Infirmary, Manchester, UK

4Department of Histopathology, Christie Hospital NHS Trust, Manchester, UK

5Cancer Research UK Department of Medical Oncology, The University of Manchester and Christie Hospital NHS Trust, Manchester, UK

6Faculty of Medicine, University of Liverpool, United Kingdom

*Corresponding Author:
Dr. Ebrahim Sakhinia
Department of Medical Genetic, Faculty of Medicine
Tabriz Molecular Analysis Centre (TGAC)
Tuberculosis and Lung Diseases research center
Tabriz university of Medical Science, Tabriz, Iran
E-mail: [email protected]

Received date: August 24, 2012; Accepted date: September 24, 2012; Published date: September 26, 2012

Citation: Sakhinia E, Goodchild J, Menasce LP, Radford J, Estiar MA, et al. (2012) Comparative Quantitation of Lymphoma Gene Signatures in Parallel Formalin Fixed Paraffin Embedded and Frozen Tissue Lymph Nodes. J Clin Exp Pathol 2:128. doi:10.4172/2161-0681.1000128

Copyright: © 2012 Sakhinia E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Abstract
Microarray gene expression profiling of frozen samples has identified Indicator genes predictive outcome in diffuse
large B-cell lymphoma (DLBCL) and follicular lymphoma (FL) and there is a now a need to translate these to use in
routine clinical material. In order to evaluate the use of Indicator genes as a diagnostic tool in routinely processed
formalin fixed paraffin embedded tissue (FFPET), we used a simple, practical polyA PCR based method for analysis
of Indicator genes in DLBCL and FL, using parallel FFPET and frozen tissue lymph nodes. PolyA PCR was used to
generate polyA cDNAs from 42 archival FFPET lymph nodes and 42 parallel frozen tissue lymph nodes. These polyA
cDNAs were assayed by real-time PCR for 35 Indicator genes, identified from previous microarray studies, predictive
of outcome in FL and DLBCL, together with housekeeping genes. Housekeeping genes were detectable at high levels
in all cases, though the 35 Indicator genes were expressed at lower levels. There was statistically significant difference
in gene expression between DLBCL and FL for one of the Indicator genes, HSP27 (p ≤ 0.02). There was no correlation
between the Indicator gene expression levels in the FFPET and frozen lymph nodes. The results demonstrate that
gene expression in FFPET is altered from that in frozen tissue and that gene expression data from frozen tissue is
therefore not transferable for use in FFPET.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords