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  • Research Article   
  • J Clin Exp Pathol 2012, Vol 2(7): 128
  • DOI: 10.4172/2161-0681.1000128

Comparative Quantitation of Lymphoma Gene Signatures in Parallel Formalin Fixed Paraffin Embedded and Frozen Tissue Lymph Nodes

Ebrahim Sakhinia1,2*, Jordan Goodchild3, Lia Patricia Menasce4, Joan Radford5, Mehrdad Asghari Estiar1, Masoud Sakhinia6 and Richard Byers3
1Department of Medical Genetic, Faculty of Medicine, Tabriz Molecular Analysis Centre (TGAC) and Tuberculosis and Lung Diseases research center, Tabriz University of Medical Sciences, Tabriz, Iran
2Division of Regenerative Medicine, School of Medicine, Faculty of Medical and Human Sciences, The University of Manchester, UK
3Department of Histopathology, Manchester Royal Infirmary, Manchester, UK
4Department of Histopathology, Christie Hospital NHS Trust, Manchester, UK
5Cancer Research UK Department of Medical Oncology, The University of Manchester and Christie Hospital NHS Trust, Manchester, UK
6Faculty of Medicine, University of Liverpool, United Kingdom
*Corresponding Author : Dr. Ebrahim Sakhinia, Department of Medical Genetics, Faculty of Medicine, Tabriz Molecular Analysis Centre (TGAC), Tuberculosis and Lung Diseases research center, Tabriz University of Medical Sciences, Tabriz, Iran, Email: [email protected]

Received Date: Aug 24, 2012 / Accepted Date: Sep 24, 2012 / Published Date: Sep 26, 2012

Abstract

Abstract
Microarray gene expression profiling of frozen samples has identified Indicator genes predictive outcome in diffuse
large B-cell lymphoma (DLBCL) and follicular lymphoma (FL) and there is a now a need to translate these to use in
routine clinical material. In order to evaluate the use of Indicator genes as a diagnostic tool in routinely processed
formalin fixed paraffin embedded tissue (FFPET), we used a simple, practical polyA PCR based method for analysis
of Indicator genes in DLBCL and FL, using parallel FFPET and frozen tissue lymph nodes. PolyA PCR was used to
generate polyA cDNAs from 42 archival FFPET lymph nodes and 42 parallel frozen tissue lymph nodes. These polyA
cDNAs were assayed by real-time PCR for 35 Indicator genes, identified from previous microarray studies, predictive
of outcome in FL and DLBCL, together with housekeeping genes. Housekeeping genes were detectable at high levels
in all cases, though the 35 Indicator genes were expressed at lower levels. There was statistically significant difference
in gene expression between DLBCL and FL for one of the Indicator genes, HSP27 (p ≤ 0.02). There was no correlation
between the Indicator gene expression levels in the FFPET and frozen lymph nodes. The results demonstrate that
gene expression in FFPET is altered from that in frozen tissue and that gene expression data from frozen tissue is
therefore not transferable for use in FFPET.

Keywords: Lymphoma; Indicator genes; Formalin fixed paraffin embedded tissue; PolyA PCR; Real-time PCR

Citation: Sakhinia E, Goodchild J, Menasce LP, Radford J, Estiar MA, et al. (2012) Comparative Quantitation of Lymphoma Gene Signatures in Parallel Formalin Fixed Paraffin Embedded and Frozen Tissue Lymph Nodes. J Clin Exp Pathol 2:128. Doi: 10.4172/2161-0681.1000128

Copyright: © 2012 Sakhinia E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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