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Diagnostic of Invasive Pulmonary Aspergillosis Using Nested PCR and Culture | OMICS International| Abstract

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  • Research Article   
  • Arch Sci 2018, Vol 2(1): 115

Diagnostic of Invasive Pulmonary Aspergillosis Using Nested PCR and Culture

Hedhili S1,2*, Souid H1,2, Baccouchi N1,2, Jemli B2,3 and Gargouri S2,3
1Faculty of Mathematical, Physical and Natural Sciences of Tunis, Tunis El Manar University, Tunisia
2Parasitology-Mycology Laboratory, Military Hospital of Tunis, , Tunisia
3Faculty of Pharmacy of Monastir, University of Monastir, Monastir, Tunisia
*Corresponding Author : Hedhili S, Faculty of Mathematical, Physical and Natural Sciences of Tunis, Tunis El Manar University, Tunisia, Tel: +21671872600, Email: [email protected]

Received Date: Mar 19, 2018 / Accepted Date: Mar 23, 2018 / Published Date: Mar 30, 2018

Abstract

A total of 120 pulmonary samples collected from 100 patients who were at high risk for invasive aspergillosis were examined by mycological test and nested PCR specific of A. fumigatus and A. flavus. Nested PCR was done using two sets of primers alp11/alp12 and alp13/alp14 coding for alkaline protease. Nineteen seven of these patients had negative PCR and culture results, two patients had nested PCR+/culture- PCR products revealed the presence of A. flavus. Thus only one patient had culture+/PCR-, morphologic identification characterized an A. niger. From 100 patients, only 3 had probable invasive pulmonary aspergillosis. Nested PCR and fungal culture correlate to 97% of case. Our data suggest that this nested PCR method may confirm and can improve the diagnosis of IPA in highrisk patients.

Keywords: Nested PCR; Mycology test; Invasive pulmonary aspergillosis

Citation: Hedhili S, Souid H, Baccouchi N, Jemli B, Gargouri S (2018) Diagnostic of Invasive Pulmonary Aspergillosis Using Nested PCR and Culture. Arch Sci 2: 115.

Copyright: © 2018 Hedhili S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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