Effect of Persistent Organic Pollutants (Dioxins) on Rat Myocardium and Amelioration with Antioxidant Vitamins (Role of Aryl Hydrocarbon Receptors and Cytochrome P450)Nashwa S Wahba1, Mona G Amer1,2*, Rehab A Karam3 and Rasha H Mohamed4
- *Corresponding Author:
- Dr. Mona Amer
Lecturer of Histology and Cell Biology
Faculty of Medicine, Zagazig university, Egypt
E-mail: mona [email protected]
Received date: September 10, 2012; Accepted date: September 24, 2012; Published date: September 26, 2012
Citation: Wahba NS, Amer MG, Karam RA, Mohamed RH (2012) Effect of Persistent Organic Pollutants (Dioxins) on Rat Myocardium and Amelioration with Antioxidant Vitamins (Role of Aryl Hydrocarbon Receptors and Cytochrome P450). J Clin Exp Pathol 2:130. doi:10.4172/2161-0681.1000130
Copyright: © 2012 Wahba NS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Epidemiologic studies have linked dioxins exposure to ischemic heart disease induced mortality. This study aimed to investigate the effect of subchronic combined exposure to tetra chloro dibenzodioxin (TCDD) and dibenzofuran (DF) on left ventricular myocardium of male rats; and the possible underlying mechanism. Moreover, this work aimed to determine the ameliorative effect of antioxidant vitamins (A, E) supplementation. Fifty animals were classified into four groups: control group (I); group (II) received vitamins only; group (III) included twenty animals received a dose of 1/10 of LD50 of combined TCDD/DF and group (IV) was included ten animals that received vitamins A and E in combination with TCDD/DF. Left ventricular myocardium was examined using histological study, immunohistochemical detection of Aryl Hydrocarbon Receptors (AHR) and mRNA expression of cytochrome P450 gene. Also, oxidant antioxidant markers in tissue homogenate were detected. Progressive cardiomyopathy, myocardial necrosis, interstitial and perivascular fibrosis were evident in a time related manner after dioxins treatment. This was associated with increased immunohistochemical expression of AHR and mRNA expression of CYP1A1. Oxidative stress was evident in left ventricle of dioxins treated group. These results suggested that combined TCDD/DF exposure has a potent cardio toxic effect which could be ameliorated by simultaneous supplementation with vitamins A and E.