Evaluating the Protective Role of Ginseng against Histological and Biochemical Changes Induced by Aflatoxin B1 in the Renal Convoluted Tubules of Adult Male Albino RatsRania A Zidan, Heba M Elnegris* and Rania A Wahdan
Department of Histology and Cell Biology, Faculty of Medicine, Zagazig University, Egypt
- *Corresponding Author:
- Elnegris HM
Department of histology and cell biology
Zagazig, University Faculty of Medicine
histology and cell biology, 21-Kawmia st
Zagazig, Sharkia, Egypt
E-mail: [email protected]
Received Date: July 29, 2015; Accepted Date: September 02, 2015; Published Date: September 04, 2015
Citation: Zidan RA, Elnegris HM, Wahdan RA (2015) Evaluating the Protective Role of Ginseng against Histological and Biochemical Changes Induced by Aflatoxin B1 in the Renal Convoluted Tubules of Adult Male Albino Rats. J Clin Exp Pathol 5:253. doi:10.4172/2161-0681.1000253
Copyright: © 2015 Zidan RA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Aflatoxins are secondary metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus. Chronic aflatoxin exposure is associated with damage to many body organs including the kidneys. Aim of the work: The aim of this study was to investigate the effect of aflatoxin B1 on the renal convoluted tubules of adult male albino rats and to determine the possible protective role of ginseng.
Materials and methods: Thirty six adult male albino rats were divided into three groups; group I: control group, group II (Aflatoxin B1 treated group): Aflatoxin B1 was administered 250 microgram/kg body weight/day, 5 days/ week for 4 weeks by oral gavages. Group III (Aflatoxin B1-Ginseng treated group): received the same dose of Aflatoxin B1 simultaneously with ginseng at a rate of 100 mg/kg body weight, by oral gavage. Blood urea nitrogen, serum creatinine and uric acid were measured. Renal cortex samples from, were processed for light and electron microscope examination.
Results: Examination of Aflatoxin B1 treated group revealed deterioration of renal functions; various degrees of renal tubular affection in the form of vacuolated tubular cells and hyaline casts in the lumen of most tubules. Some tubular cells had small darkly stained nuclei while others had sloughed epithelial cells in their lumina. Weak positive cytoplasmic reaction of Bcl2 was detected in most renal tubular cells. These changes were markedly reduced with administration of ginseng in group III.
Conclusion: Aflatoxin leads to alterations in the histological structure and functions of renal convoluted tubules of albino rats and ginseng supplementation could be protective from these changes.