GLIPR1 Regulates TIMP1-CD63-ITGB1-AKT Signaling Pathway in Glioma Cells and Induces Malignant Transformation of Astroglioma
Received Date: Sep 21, 2021 / Accepted Date: Oct 12, 2021 / Published Date: Oct 19, 2021
Abstract
Aim: The new diagnostic and prognostic tumor markers related to astrocytoma (ACM) was found to improve the diagnosis rate, reduce the poor prognosis.
Methods: The activity of SHC-44 and SW1783 cells under regulation of GLIPR1 was investigated by CCK8 analysis. The effect of GLIPR1 on the proliferation of SHC-44 and SW1783 cells was analyzed by Cell colony-forming experiment. The migration of SHC-44 and SW1783 cells under regulation of GLIPR1 was analyzed by Transwell assay. The effect of GLIPR1 on the invasion of SHC-44 and SW1783 cells was analyzed by Cell scratch test. Immunofluorescence was employed to analyze the expression characteristics of GLIPR1 and CD63 proteins. The effects of GLIPR1 on the protein expression of GLIPR1, TIMP1, CD63, ITGB1 and AKT in SHC-44 and SW1783 cells was analyzed by Western blot.
Results: The outcomes revealed that GLIPR1 could enhance the activity, proliferation, migration and invasion of ACM cells, which might be associated with the activation of TIMP1-CD63-ITGB1-AKT signaling pathway.
Conclusion: Taken together, GLIPR1 might be a potential target for the prevention or management of ACM in the clinic.
Keywords: GLIPR1, Astroglioma, Proliferation, Migration, Invasion, CD63
Citation: Li F, Zhang W, Wang M, Jia P (2021) GLIPR1 Regulates TIMP1- CD63-ITGB1-AKT Signaling Pathway in Glioma Cells and Induces Malignant Transformation of Astroglioma. Cell Mol Biol 67: 212. Doi: 10.4172/1165-158X.1000212
Copyright: © 2021 Li F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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