alexa Improved Method for Estimating M-Spike Proteins in Seru
ISSN: 2161-0681

Journal of Clinical & Experimental Pathology
Open Access

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Review Article

Improved Method for Estimating M-Spike Proteins in Serum Protein Electrophoresis

Zhang S, Wu XX, Ostrovsky I and Rand JH*

Montefiore Medical Center, Bronx, New York, USA

*Corresponding Author:
Jacob H. Rand
Director, Pathology Department, Hematology Laboratories
Montefiore Medical Center, Bronx, New York, USA
Tel: 001-718-920-5991
E-mail: [email protected]

Received Date: March 25, 2014; Accepted Date: June 24, 2014; Published Date: June 26, 2014

Citation: Zhang S, Wu XX, Ostrovsky I, Rand JH (2014) Improved Method for Estimating M-Spike Proteins in Serum Protein Electrophoresis. J Clin Exp Pathol 4:178. doi:10.4172/2161-0681.1000178

Copyright: © 2014 Zhang S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

 

Abstract

Context: Serum monoclonal immunoglobulin (M-spike) in multiple myeloma is measured by gel electrophoresis (SPE) followed by densitometric scanning of the gel. The current standard methods delimit the M-spike component based on the projected gel image on screen (current standard method, CSM). However, the M-spike could also be selected by delimiting the peak(s) in the scanned curve (densitometry based method, DBM).

Objective: The current study will correlate the results with these two approaches and to investigate which method may yield a result more close to the actual M-spike in the serum.

Designs: Forty-one consecutive SPE files from 2010-2011 with positive M-spike were analyzed simultaneously with methods CSM and DBM. Serum monoclonal IgG from a myeloma patient with essentially no background polyclonal immunoglobulins was purified using protein G sepharose column and quantified using UV spectrophotometry. The measured concentration of purified IgG using methods CSM and DBM was compared to true IgG levels in spiked samples.

Results: The measurements of 41 M-spikes using methods CSM and DBM correlated significantly (r=0.988, p<0.01). However, the measurement using method DBM was consistently higher than that using method CSM (49% ± 24%) In the measurement of purified monoclonal IgG, compared to method CSM, method DBM gave consistently closer results to the true IgG levels in spiked samples.

Conclusions: The current method (CSM) underestimates the amount of serum M-spike. The revised method (DSB) based on the densitometric peak more accurately reflects serum M-spike levels using SPE.

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