Improved Method for Estimating M-Spike Proteins in Serum Protein Electrophoresis | OMICS International| Abstract
ISSN: 2161-0681

Journal of Clinical & Experimental Pathology
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
  • Review Article   
  • J Clin Exp Pathol 2014, Vol 4(4): 178
  • DOI: 10.4172/2161-0681.1000178

Improved Method for Estimating M-Spike Proteins in Serum Protein Electrophoresis

Zhang S, Wu XX, Ostrovsky I and Rand JH*
Montefiore Medical Center, Bronx, New York, USA
*Corresponding Author : Rand JH, Director, Pathology Department, Hematology Laboratories, Montefiore Medical Center, Bronx, New York, USA, Tel: 001-718-920-5991, Email: [email protected]

Received Date: Mar 25, 2014 / Accepted Date: Jun 24, 2014 / Published Date: Jun 26, 2014


Context: Serum monoclonal immunoglobulin (M-spike) in multiple myeloma is measured by gel electrophoresis (SPE) followed by densitometric scanning of the gel. The current standard methods delimit the M-spike component based on the projected gel image on screen (current standard method, CSM). However, the M-spike could also be selected by delimiting the peak(s) in the scanned curve (densitometry based method, DBM).

Objective: The current study will correlate the results with these two approaches and to investigate which method may yield a result more close to the actual M-spike in the serum.

Designs: Forty-one consecutive SPE files from 2010-2011 with positive M-spike were analyzed simultaneously with methods CSM and DBM. Serum monoclonal IgG from a myeloma patient with essentially no background polyclonal immunoglobulins was purified using protein G sepharose column and quantified using UV spectrophotometry. The measured concentration of purified IgG using methods CSM and DBM was compared to true IgG levels in spiked samples.

Results: The measurements of 41 M-spikes using methods CSM and DBM correlated significantly (r=0.988, p<0.01). However, the measurement using method DBM was consistently higher than that using method CSM (49% ± 24%) In the measurement of purified monoclonal IgG, compared to method CSM, method DBM gave consistently closer results to the true IgG levels in spiked samples.

Conclusions: The current method (CSM) underestimates the amount of serum M-spike. The revised method (DSB) based on the densitometric peak more accurately reflects serum M-spike levels using SPE.

Keywords: Serum monoclonal immunoglobulin; Serum protein electrophoresis; M-spike proteins

Citation: Zhang S, Wu XX, Ostrovsky I, Rand JH (2014) Improved Method for Estimating M-Spike Proteins in Serum Protein Electrophoresis. J Clin Exp Pathol 4:178. Doi: 10.4172/2161-0681.1000178

Copyright: © 2014 Zhang S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.