Abstract

By crossing Ae, a collection of Aegilops tauschii-wheat introgression (A-WI) lines were created to break the wheat D genome's narrow diversity bottleneck tauschii promotion T015 with normal wheat first class cultivar Zhoumai 18 (Zhou18). Based on Single Nucleotide Polymorphism (SNP) markers, a high-density genetic map was created, and 15 yield-related traits were tested in 11 environments to find quantitative trait loci (QTL). In at least five environments, a total of 27 environmentally stable QTLs were discovered, 20 of which were derived from Ae. up to 24.27 percent of the phenotypic variations can be accounted for by tauschii T015. Based on nonsynonymous nucleotide mutations in the candidate gene AetT093-2Dv1G100900, three successful Kompetitive Allele Specific PCR (KASP) markers demonstrated that A-WI lines with the T015 haplotype had significantly longer. For breeding improvement, four primary valuable A-WIs with good trait performance and yield-related QTL were chosen. The outcomes will make it easier to transfer beneficial genes from Ae in an effective manner. tauschii into wheat cultivars to further develop wheat yield and different characteristics.