alexa Interferon Regulatory Factor 6 (IRF6) and Gene and#8211; Environment Interactions in Non-Syndromic Orofacial Cleft Cases in Saudi Arabia-A Case Control Study| Abstract

ISSN: 2332-0702

Journal of Oral Hygiene & Health

  • Research Article   
  • J Oral Hyg Health, Vol 6(1): 233
  • DOI: 10.4172/2332-0702.1000233

Interferon Regulatory Factor 6 (IRF6) and Gene – Environment Interactions in Non-Syndromic Orofacial Cleft Cases in Saudi Arabia-A Case Control Study

Najlaa M Alamoudi1*, Heba J Sabbagh1,2, Nicola P Innes2, Sherif Edris Ahmed3, Azeez Butali4, Eman Abdulbaset Alnamnakani5, Sari Rabah6, Mustafa A Hamdan7, Nasir H Alhamlan8, Fatma D Abdulhameed9, Najat M Farsi1, Ali H Hassan10 and Peter A Mossey11
1Pediatric Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia
2Paediatric Dentistry, University of Dundee Dental School, , Dundee, Scotland, UK
3Princess Al-Jawhara Al-Brahim Centre of Excellence in Research of Hereditary Disorders, , Jeddah, Saudi Arabia
4Department of Oral Pathology, College of Dentistry, Radiology and Medicine/ Dows Institute for Dental Research, University of Iowa, USA
5Riyadh Military Hospital, , Prince Sultan Military Medical City, Riyadh, Saudi Arabia
6Surgery Department, King Abdullah bin Abdulaziz University Hospital, Princess Noura University and King Saud bin Abdulaziz University for Health Sciences, , Riyadh, Saudi Arabia
7Plastic Surgery Department, , King Saud Medical City, Ministry of Health, Riyadh, Saudi Arabia
8King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia
9Pediatric Surgery Department, Madina Maternity and Children’s Hospital, , Saudi Arabia
10Orthodontic Department, King Abdulaziz University, Jeddah, Saudi Arabia
11Division of Oral Health Sciences and WHO Collaborating Centre for Oral Health and Craniofacial, University of Dundee Dental School, , Dundee, Scotland, UK
*Corresponding Author : Najlaa M Alamoudi, Faculty of Dentistry, Pediatric Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia, Tel: 6401000 (22026-20388-22264), Email: [email protected]

Received Date: Oct 18, 2017 / Accepted Date: Dec 11, 2017 / Published Date: Jan 11, 2018

Abstract

The association between interferon regulatory factor 6 (IRF6) and nonsyndromic orofacial cleft (NSOFC) is affected by ethnicity. Also, gene-environment interactions (GEI) may play an important role in its etiology.
Objectives: This case-control study investigated whether IRF6 gene variants were associated with NSOFC in Saudi Arabian population and whether the gene was affected by maternal environmental exposures.
Methods: We extracted DNA from saliva samples obtained from 171 infant–parent triad cases and 189 matched controls (age, gender, and location) from January 2010–December 2011; this study included a total of 11 referral hospitals in Saudi Arabia. IRF6 (rs2013162, rs2235375, and rs2235371) polymorphisms were genotyped using restriction-digestion polymerase chain reaction. Data on environmental exposures, for GEI analyses, were collected through questionnaire-led interviews with parents.
Results: We found statistically significant over transmission of the common IRF6 rs2013162 allele among cleft lip with or without palate CL(P) cases. No associations were found for either of the other two IRF6 SNPs. Maternal exposure to antipyretics, folic acid, fever, antibiotics, illnesses, common cold/flu, paternal water pipe smoking, stress, x-rays, and/or chemicals could significantly interact with the maternal IRF6 (rs2013162 and rs2235375) gene variants, affecting the likelihood of having an offspring with NSOFC.
Conclusion: The common allele at IRF6 rs2013162 was significantly over transmitted among CL(P) cases. This study provides hypotheses for future investigations into genetic and environmental factors and their interaction in the etiology of NSOFC.

Keywords: Interferone regulatory factor 6; Nonsyndromic orofacial cleft; Cleft lip and palate; Gene-environment interactions; aetiology; Cleft lip with or without palate

Citation: Alamoudi NM, Sabbagh HJ, Innes NP, Ahmed SE, Butali A, et al. (2018) Interferon Regulatory Factor 6 (IRF6) and Gene–Environment Interactions in Non- Syndromic Orofacial Cleft Cases in Saudi Arabia-A Case Control Study. J Oral Hyg Health 6: 233. Doi: 10.4172/2332-0702.1000233

Copyright: © 2018 Alamoudi NM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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