ISSN: 2155-6199

Journal of Bioremediation & Biodegradation
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  • Research Article   
  • J Bioremediat Biodegrad 9:440,
  • DOI: 10.4172/2155-6199.1000440

Isolation and Characterization of Filter Paper Degrading Bacteria from the Guts of Coptotermes formosanus

Egwuatu TF1* and Appeh OG2
1Department of Cell Biology and Genetics, Faculty of Science, University of Lagos, Akoka, Nigeria
2Michael Okpara University of Agriculture, Umudike, Umuahia, Abia State, Nigeria
*Corresponding Author : Egwuatu TF, Department of Cell Biology and Genetics, Faculty of Science, University of Lagos, Akoka, Nigeria, Tel: +2348060017071, Email: tochukwufrankegwuatu@gmail.com

Received Date: Apr 20, 2018 / Accepted Date: Apr 30, 2018 / Published Date: May 03, 2018

Abstract

Background: The filter paper utilizing capabilities of Pseudomonas mendocina, Burkholderia pseudomallei, Chryseobacterium luteola, Klebsiella oxytoca and Klebsiella terrigena isolated from the gut of a local termite Coptotermes formosanus were analysed.
Design and methods: The isolates were inoculated into a buffered medium containing minerals and Whatman filter paper as sole source of carbon to observe the ability of these bacteria to digest solid substrate. The ability of the isolates to grow in this medium as well as to digest the filter paper was determined by visual observation after 30 days. Reducing sugar test and gravimetric analysis were also carried out at the end of 30 days.
Results: All bacteria cultures showed growth as the medium turned cloudy and the filter paper became macerated. The gravimetric analysis of the residual filter in the liquid medium at the end of 30 days incubation showed that Chryseobacterium luteola had the highest degradation rate of 95%, Pseudomonas mendocina had the degradation rate of 90%, whilst Burkholderia pseudomallei, Klebsiella oxytoca and Klebsiella terrigena had biodegradation rate of 75% each. Reducing sugar test and paper chromatography carried out for glucose production were positive showing their ability to convert cellulose to glucose.
Conclusion: The bacterial isolates showed a potential to convert cellulose into reducing sugars which could be readily used in many applications like feed stock for production of valuable organic compounds; for example in simultaneous saccharification and fermentation of cellulose into ethanol.

Keywords: Biodegradation; Chromatography; Amplification; Cloudy; Cellulose

Citation: Egwuatu TF, Appeh OG (2018) Isolation and Characterization of Filter Paper Degrading Bacteria from the Guts of Coptotermes formosanus. J Bioremediat Biodegrad 9: 440. Doi: 10.4172/2155-6199.1000440

Copyright: © 2018 Egwuatu TF, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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