alexa KLF4 Improve Prognosis of Triple-negative Breast Cancer by Suppression of Epithelial-mesenchymal Transition | OMICS International| Abstract
ISSN: 2572-4118

Breast Cancer: Current Research
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  • Research Article   
  • Breast Can Curr Res, Vol 1(3): 110
  • DOI: 10.4172/2572-4118.1000110

KLF4 Improve Prognosis of Triple-negative Breast Cancer by Suppression of Epithelial-mesenchymal Transition

Takuya Nagata1*, Katsuo Shimada2, Lv Xiao Long1, Tomoyuki Okumura1, Kenichi Tazawa3, Koichi Higashiyama4, Tetsuro Shimzu5 and Takashi Ohnaga6
1Department of Surgery and Science, Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, 2630 Sigitani Toyama 930-0194,, Japan
2Department of Surgery, Imizu Municipal Hospital, , 20 Honoki Imizu Toyama 934-0053, Japan
3Department of Surgery, Itoigawa General Hospital, , 457-1 Takegahana Itoigawa Niigata, 941-8502, Japan
4Department of Surgery, Asahi General Hospital, , 477 Tomari Asahi Simoniikawa, 939-0798, Japan
5Department of Surgery, Saiseikai Toyama Hospital, , 33-1, Kusunoki Toyama, 931-8533, Japan
6Central Research Laboratories, Toyama Industrial Technology Center, , 150 Futagami-cho, Takaoka, Toyama 933-0981, Japan
*Corresponding Author : Takuya Nagata, Department of Surgery and Science, Graduate School of Medicine and Pharmaceutical Sciences for Research, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan, Tel: +81-76-434-7331, Email: [email protected]

Received Date: Jun 18, 2016 / Accepted Date: Jul 14, 2016 / Published Date: Jul 28, 2016

Abstract

Background: The prognostic value of KLF4 in triple negative breast cancer (TNBC) was described. Here we report an analysis of KLF4 expression in peripheral blood circulating tumor cells (CTCs) from patients with TNBC, which provided evidence that KLF4 negatively regulates the metastasis and growth of TNBC.

Method: We assessed the expression levels of KLF4 in 84 patients with TNBC by immunohistochemical staining and studied the patterns of metastasis/recurrence clinicopathologically. In addition, CTCs in the peripheral blood of TNBC patients were identified and compared with primary lesions in terms of KLF4 expression. Moreover, the expression of KLF4 was inhibited by transfecting cultured TNBC cells (MDA-MB231) with the small interfering RNA (siRNA) of KLF4 to analyze the effects of KLF4 on cell proliferation and epithelial-mesenchymal transition (EMT) -like changes.

Results: In the 84 patients with TNBC, higher KLF4 expression was associated with significantly better overall survival (OS) and disease-free survival (DFS). An analysis of KLF4 expression in CTCs of the TNBC patients showed that KLF4 expression was lower in CTCs than in cancer cells in primary lesions. TNBC cells (MDA-MB231) with inhibited KLF4 expression exhibited a greater ability to growth than controls. These cells also underwent EMT-like changes with reduced expression of epitherial factors such as E-cadherin. Treating these TNBC cells with eribulin resulted a reduction of the expression of stem cell/EMT markers.

Conclusion: TNBC patients with reduced KLF4 expression had poor outcomes. The results of our experiments suggest the expression of KLF4 is one of the important factors that inhibit the EMT and growth of TNBC.

Keywords: Triple negative breast cancer; KLF4; Circulating tumor cell; Epithelial-mesenchymal transition; iPS cells-induced pluripotent stem cells; Antibody; Overall survival; Disease free survival; Small interfering RNA

Citation: Nagata T, Shimada K, Long XL, Okumura T, Tazawa K, et al. (2016) KLF4 Improve Prognosis of Triple-negative Breast Cancer by Suppression of Epithelial-mesenchymal Transition. Breast Can Curr Res 1: 110. Doi: 10.4172/2572-4118.1000110

Copyright: ©2016 Nagata T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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