Abstract

Abstract
Endogen intoxication trigger in acute hepatitis C is hepatic cells cytolysis caused by viral reproduction with
subsequent immune system reaction. All above mentioned needs further study of formation mechanisms in Chronic
Hepatitis C (CHC) and motivation of immunosuppressive therapy. So this research aim was to determine the monitoring
metabolic indexes state in patients with chronic viral hepatitis C and motivate such diagnostic criteria as significant
tests for controlling the endogen intoxication.
Materials and methods: For this purpose, in serum of patients with hepatitis C and conventionally healthy people
from control group the activity of enzymes were detected. To study the bioenergetics changes in serum lactate/pyruvate
and NAD/NADH+ correlation were investigated. Mentioned indexes detection was performed using photometric method
with a set of chemicals manufactured by firm “Filisit-Diagnosticum”, Ukraine. For determination of lactate and pyruvate
test kits produced by “Olveks”, Ukraine, were used according to manufacturer’s recommendations.
Results and discussion: Thus, the results of monitoring indexes study in patients with CHC have found multiple
organ disorders of metabolic processes based on membrane pathology. Inhibition of reduction synthesis bioenergetics
in human body and catabolic processes activation which are connected with the tension of many organs and tissues
that in turn leads to endogenous intoxication. That’s why monitoring indexes state determination is important in
evaluation of the course severity degree at CHC according to the level of endogen intoxication.

Keywords: Endogen intoxication; NAD/NADH⁺; Chronic hepatitis C