alexa miR-21 Functions Oppositely in Proliferation and Differentiation of Neural Stem/Precursor Cells via Regulating AKT and GSK-3andbeta; | OMICS International| Abstract

Cellular and Molecular Biology
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
  • Research Article   
  • Cell Mol Biol 2016, Vol 62(4): 145
  • DOI: 10.4172/1165-158X.1000145

miR-21 Functions Oppositely in Proliferation and Differentiation of Neural Stem/Precursor Cells via Regulating AKT and GSK-3β

Xisong Gao1, Xiaoning Li1, Chao Qian1, Feng Li1, Yu Zhang1, Lianfeng Dang1, Xueqian Xiao1, Feng Liu1, Haima Li1 and Xiujuan Zhang2*
1Hospital of Shaanxi Nuclear Industry, , P.R. China
2Xi’an No.3 Hospital, , Fengcheng Road, Xi’an, Shaanxi 710021, P.R. China
*Corresponding Author : Xiujuan Zhang, Xi’an No. 3 Hospital, 10 Fengcheng Road, Xi’an, Shaanxi 710021, P.R. China, Tel: +86-2933577783, Email: [email protected]

Received Date: Sep 13, 2016 / Accepted Date: Dec 08, 2016 / Published Date: Dec 13, 2016


MicroRNA involves in regulating behavior of neural stem/precursor cells (NSPCs), thus it offers the potential to treat central nervous system disease. However, the effect of miR-21 on NSPCs remains unknown. In this study, we demonstrated that miR-21 reduced proliferation and promoted neural differentiation in NSPCs via regulating the activation of AKT and GSK-3β signaling pathways in vitro. During differentiation of NSPCs, the expression of miR-21 was increased in a time-dependent manner by qRT-PCR. Synthesized pre-miR-21 or anti-miR-21 was transfected into NSPCs, thereby efficiently over-expressing or knocking down miR-21. Overexpression of miR-21 promoted the neural differentiation of NSPCs, as indicated by Tuj1 and PSA-NCAM staining. Interestingly, knocking down miR-21 had the opposite effect of neural differentiation in NSPCs. However, in proliferation area, overexpression of miR-21 decreased the cell viability by 3-((2-methyl-1,3-thiazol-4-yl)ethynyl)pyridine hydrochloride (MTT) assay, and inhibited the proliferation of NSPCs, as indicated by 5-Bromo-2-deoxyUridine (BrdU) staining. And likewise, knocking down miR-21 had the opposite effect of cell viability and proliferation. Western blot showed that overexpression of miR-21 enhanced the expression of Cyclin D1; however, knocking down miR-21 prevented its expression. Furthermore, we revealed that protein kinase B (AKT) and glycogen synthase kinase-3 beta (GSK-3β) signaling pathways were involved in the proliferation and neural differentiation of NSPCs. Overexpression of miR-21 activated AKT, and the p-GSK-3β was increased. Conversely, knocking down miR-21 blocked the activation of AKT, and decreased the phosphorylation level of GSK-3β. These results demonstrated that miR-21 promotes neural differentiation and reduces proliferation in NSPCs via regulating AKT and GSK-3β pathways. These findings may help to develop strategies for treatment of central nervous system diseases.

Keywords: Neural stem/precursor cells; miR-21; Proliferation; Differentiation

Citation: Gao X, Li X, Qian C, Li F, Zhang Y, et al. (2016) miR-21 Functions Oppositely in Proliferation and Differentiation of Neural Stem/Precursor Cells via Regulating AKT and GSK-3ß. Cell Mol Biol 62:145. Doi: 10.4172/1165-158X.1000145

Copyright: © 2016 Gao X, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Select your language of interest to view the total content in your interested language

Post Your Comment Citation
Share This Article
Recommended Conferences
Article Usage
  • Total views: 301
  • [From(publication date): 0-2016 - Jan 23, 2020]
  • Breakdown by view type
  • HTML page views: 261
  • PDF downloads: 40