Molecular Characterization of Culex theileri from Canary Islands, Spain, a Potential Vector of Dirofilaria immitis | OMICS International| Abstract
ISSN: 2161-0681

Journal of Clinical & Experimental Pathology
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  • Research Article   
  • J Clinic Experiment Pathol 2012, Vol 2(2): 001
  • DOI: 10.4172/2161-0681.S3-001

Molecular Characterization of Culex theileri from Canary Islands, Spain, a Potential Vector of Dirofilaria immitis

Morchón R1*, Bargues M D2, Latorre-Estivalis J M2, Pou-Barreto C3, Melero-Alcibar R5, Moreno M4, Valladares B3, Molina R5, Montoya- Alonso J A4, Mas-Coma S2 and Simón F1
1Laboratorio de Parasitologia, Facultad de Farmacia, Universidad de Salamanca, Av. del Campo Charro s/n, 37007 Salamanca, Spain
2Departamento de Parasitologia, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andres Estelles s/n, 46100 Burjassot, Valencia, Spain
3Instituto Universitario de Enfermedades Tropicales y Salud Publica de las Islas Canarias. Universidad de La Laguna, 38271, Tenerife, Spain
4Departamento de Medicina Interna, Facultad de Veterinaria, Universidad de Las Palmas de Gran Canaria, Las Palmas, Spain
5Servicio de Parasitologia, Centro Nacional de Microbiologia, ISCIII, Ctra. Majadahonda - Pozuelo s/n, 28220 Majadahonda, Madrid, Spain
*Corresponding Author : Morchón R, University of Salamanca, Faculty Of Pharmacy, Laboratory Of Parasitology, Avda Campo Charro S/n, 37007 Salamanca, Spain, Tel: +34923294535, Fax: +34923294515, Email: [email protected]

Received Date: Oct 20, 2011 / Accepted Date: Dec 03, 2011 / Published Date: Dec 06, 2011


Dirofilaria immitis is the causal agent of heartworm diseases and of human pulmonary dirofilariosis. The infection
is transmitted by several species of culicid mosquitoes that are frequently able to bite both animal reservoirs and
humans. Canary Islands (Spain) constitute a well documented endemic area of canine dirofilariosis in which the
mosquito species involved in the transmission of D. immitis are not known. The objectives of the present work were
the identification of vectors of this parasite in Canary Islands and their molecular characterization. A total of 1219
female mosquitoes were captured. The most abundant species was Culex theileri (52.26%) followed by Cx. pipiens
(35.44%), Anopheles cinereus hispaniola (6.23%), Culiseta longiareolata (5.74%), and Culex laticintus (0.33%). PCR
was applied for the detection of larval D. immitis DNA in mosquitoes. D. immitis DNA was observed in the abdomen of
one Cx. theileri female: 0.082% of the entire mosquito population and 0.17% in Cx. theileri. A molecular identification
of Cx. theileri, the potential mosquito vector of dirofilariosis in this zoonotic focus in Canary Islands of Spain, has
been made for first time based on sequences of the 18S rRNA gene, the second internal transcribed spacer (ITS2)
of ribosomal DNA and the barcode region of the cytochrome c oxidase I (cox1) gene of mitochondrial DNA, allowing
a broad mosquito molecular basis for future populations genetic analyses of this vector species. Parasitological and
entomological molecular results suggest that Cx. theileri is a potential natural vector of D. immitis in Canary Islands.

Keywords: Dirofilaria immitis; Culex theileri; Canary Islands; Spain; 18S rRNA gene; ITS-2 rDNA; Barcode region mtDNA

Citation: Morchón R, Bargues MD, Latorre-Estivalis JM, Pou-Barreto C, Melero- Alcibar R, et al. (2011) Molecular Characterization of Culex Theileri from Canary Islands, Spain, a Potential Vector of Dirofilaria Immitis. J Clinic Experiment Pathol S3:001. Doi: 10.4172/2161-0681.S3-001

Copyright: © 2011 Morchón R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Review summary

  1. gera Malben
    Posted on Apr 09 2016 at 4:48 am
    Hola buen día , quisiera saber la clasificación mas actual de los nematodos en veterinaria en especial de la Dirofilaria immitis. gracias Atte MVZ Malben