Monitoring of Molecular Variation among Egyptian Faba Bean Rhizobium Isolates as Response to Pesticides Stress
Received Date: Mar 26, 2015 / Accepted Date: May 27, 2015 / Published Date: May 29, 2015
Abstract
Ten Rhizobium isolates from nodules of Faba bean (Vicia faba L.) plants that represent different geographic and climatic locations in Egypt were evaluated for their tolerance against different doses of Zeus and Stomp as pesticides (250, 500 and 750 mg). In general, with increasing of pesticides doses, the tolerance and the viability of isolates were decreased. All isolates did not succeed to grow at the highest dose of Zeus (750 mg), while the isolates RLA, RLG and RLI succeeded to grow at the same concentration of Stomp. The most important nodulation genes nodA, nodD and the bidirectional promoter region between them were amplified using PCR and two principals bands were detected in the most isolates. The protein banding patterns of some Rhizobium isolates were detected using SDSPAGE analysis under pesticides stress and compared with their untreated control.
Keywords: Rhizobium; Legumes; Nod D - A genes and Pesticides
Citation: Abdel-lateif KS, El-Zanaty AFM, Hewedy OA, El Sobky MA (2015) Monitoring of Molecular Variation among Egyptian Faba Bean Rhizobium Isolates as Response to Pesticides Stress. J Bioremed Biodeg 6: 296. Doi: 10.4172/2155-6199.1000296
Copyright: © 2015 Abdel-lateif KS, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permitsunrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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