MUC1 and C-Met Affect Proliferation, Intercellular Junctions and Invasion in Two Head and Neck Carcinoma Cell Lines
Received Date: Nov 10, 2017 / Accepted Date: Nov 23, 2017 / Published Date: Nov 27, 2017
Abstract
Role of metalloproteases and adhesion molecules has been studied in cancer and metastases; tyrosine kinase receptors (TKRs) and mucins are related to their expression.
Objective: To investigate the effects of MUC1/c-Met, and their participation in metastatic mechanism in head and neck carcinoma cell lines.
Materials and methods: Lines Cal27 and A253 from squamous cell carcinoma and submaxillary gland carcinoma were treated with SU11274 (c-Met inhibitor) and GO-201 (MUC1 inhibitor) and evaluated by western blot and immunocytochemistry with anti-claudin 1, 3, and 9, integrin-αVβ1, E-cadherin, MMP2, MMP9, TIMP1 and TIMP2 after inhibition. MMPs’ activity was assessed by zymography.
Results: Claudins were atypically located in the cytoplasm and nucleus and their expression is differentially modified. Migration and invasion rate were affected by inhibition. MMP9 activity was affected.
Conclusion: Our results suggest that the role of regulating MUC1 and c-Met is related to invasion mechanisms by dysregulation of claudins and MMPs activity.
Keywords: Head and neck carcinoma; Invasion; c-Met; MUC1; Claudins
Citation: Amador CV, Rodríguez AC, Zenteno E, Guerrero JCH, Farfán MDJ (2017) MUC1 and C-Met Affects Proliferation, Intercellular Junctions and Invasion in Two Head and Neck Carcinoma Cell Lines. J Clin Exp Pathol 7: 326. Doi: 10.4172/2161-0681.1000326
Copyright: ©2017 Amador CV, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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