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Phytochemical Screening and <em>In Vitro</em> Antitrypanosomal Activity of Aqueous and Methanol Leaf Extract of <em>Verbascum sinaiticum </em>(Scrophulariaceae) against <em>Trypanosoma congolense</em> Field Isolate | Abstract
ISSN: 2161-0681

Journal of Clinical & Experimental Pathology
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Research Article

Phytochemical Screening and In Vitro Antitrypanosomal Activity of Aqueous and Methanol Leaf Extract of Verbascum sinaiticum (Scrophulariaceae) against Trypanosoma congolense Field Isolate

Ermias Mergia1*, Workineh Shibeshi2, Getachew Terefe3 and Tilahun Teklehaymanot4

1Department of Pharmacy, Faculty of Health Sciences, Rift Valley University College, Ethiopia

2Department of Pharmacology and Clinical Pharmacy, Faculty of Health Sciences, Addis Ababa University, Ethiopia

3Department of Parasitology, Faculty of Veterinary Medicine, Addis Ababa University, Ethiopia

4Endod and Other Medicinal Plants Unit, Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Ethiopia

*Corresponding Author:
Ermias Mergia
Department of Pharmacy, Faculty of Health Sciences
Rift Valley University College, Ethiopia
Tel: 251911363614
E-mail: [email protected]

Received Date: May 19, 2014; Accepted Date: July 07, 2014; Published Date: July 09, 2014

Citation: Mergia E, Shibeshi W, Terefe G, Teklehaymanot T (2014) Phytochemical Screening and In Vitro Antitrypanosomal Activity of Aqueous and Methanol Leaf Extract of Verbascum sinaiticum (Scrophulariaceae) against Trypanosoma congolense Field Isolate. J Clin Exp Pathol 4:183. doi: 10.4172/2161-0681.1000183

Copyright: © 2014 Mergia E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Aqueous and methanol leaf extracts of V. sinaiticum were investigated for the presence of secondary metabolites and their in vitro activity against Trypanosoma congolence, the main causative agent of African animal trypanosomosis in Sub-Saharan Africa and Ethiopia. The in vitro assay was carried out by monitoring test concentrations of 4, 2, 1, 0.4 and 0.2 mg/ml for cessation or reduction in motility of trypanosomes followed by monitoring for loss of infectivity to mice. Phytochemical screening revealed presence of alkaloids, flavonoids, glycosides, phenolic compounds, saponins, steroids and tannins. An appreciable in vitro activity was attained by the methanol extract of V. sinaiticum at 4 mg/ml concentration. In general, the results obtained suggest ethnopharmacological usefulness of the plant and necessitate further studies to be carried on isolated active substances from the plant.


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