ISSN: 2475-7640

Journal of Clinical and Experimental Transplantation
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
  • Mini Review   
  • J Clin Exp Transplant 2017,
  • DOI: 10.4172/2475-7640.1000112

Technical Modifications to Improve Islet Yield from Chronic Pancreatitis Pancreas (CPP) for Islet Auto-Transplantation (IAT)

Maheswaran Dhanasekaran1, Gopalakrishnan Loganathan1, Siddharth Narayanan1, William Tucker, Aravinth Prasanth Jawahar1, Ankit Patel1, Venugopal Subhashree2, Sri Prakash Mokshagundam1, Stuart K Williams1, Michael J Hughes1 and Appakalai N Balamurugan1*
1Department of Surgery, Centre for Cellular Transplantation, Cardiovascular Innovation Institute, University of Louisville, Louisville, KY, USA
2School of Biosciences and Technology, VIT University, Vellore, TN, India
*Corresponding Author : Appakalai N Balamurugan, Department of Surgery, Clinical Islet Cell Laboratory Centre for Cellular Transplantation, Cardiovascular Innovation Institute, University of Louisville, Louisville, KY, USA

Received Date: Dec 16, 2016 / Accepted Date: Jan 30, 2016 / Published Date: Feb 06, 2017

Abstract

Surgical removal of the pancreas in patients with intractable chronic pancreatitis will potentially mitigate the long standing suffering of abdominal pain and improve the quality of life. Nonetheless, the induction of iatrogenic diabetes from surgery becomes irrevocable as the insulin producing islet cells are removed. This novel process of islet isolation (both auto- and allo-) involves specific procurement and packaging of the pancreas which is then transported to a specialized clean-room where islets are isolated. The isolation involves distending the pancreas with dissociation enzymes that help in digesting the extracellular matrix and freeing the islets. The cells are then collected as pellets and purified if necessary by density gradient and later packed in the transplant bag for intra-portal infusion back to the patient. The expectant metabolic outcome following the procedure is solely dependent on the total islet mass transplanted. Despite incorporating the most standardized isolation techniques, the overall islet yields available from the diseased pancreas are still very low. Factors such as donor characteristics, pancreatic fibrosis and cellular atrophy further reduce the yield highlighting the need for effective islet isolation techniques.

Keywords: Chronic pancreatitis; Pancreatectomy; Autograft; Allograft; Human islets; Autologous islet isolation; Transplantation; Insulin independence; Collagenase digestion

Citation: Dhanasekaran M, Loganathan G, Narayanan S, Tucker W, Patel A, et al. (2017) Technical Modifications to Improve Islet Yield from Chronic Pancreatitis Pancreas (CPP) for Islet Auto-Transplantation (IAT). J Clin Exp Transplant 2: 112. Doi: 10.4172/2475-7640.1000112

Copyright: © 2017 Dhanasekaran M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Top