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  • Research Article   
  • Cell Mol Biol; 2022, Vol 68(2): 227
  • DOI: 10.4172/1165-158X.1000227

The Study of the Influence of Unfolded Protein Response and Autophagy on Sorafenib-Induced Tumor Inhibition of Hepatocellular Carcinoma

Jian Wu1#, Qi Zhuang2#, Xiaojie Gao3, Xiongwei Yang3, Rili Qin3, Liying Zhang4* and Xinchen Zhang3*
1Department of Anesthesiology, The 960th Hospital of the People's Liberation Army, No. 20, Zhanbei Road, Zibo 255300, Shandong, China
2Department of General Surgery, The First Hospital of Qiqihar City, 30 Gongyuan Road, Qiqihar 161005, Heilongjiang, China
3Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, 246 Xue fu Road, Nangang District, Harbin 150086, Heilongjiang, China
4Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Harbin Medical University, 246 Xue fu Road, Nangang District, Harbin 150086, Heilongjiang, China
#Contributed equally to this work
*Corresponding Author (s) : Liying Zhang, Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Harbin Medical University, 246 Xue fu Road, Nangang District, Harbin 150086, Harbin 150086, Heilongjiang, China, Email: lyzly1978@hrbmu.edu.cn
Xinchen Zhang, Department of General Surgery, The Second Affiliated Hospital of Harbin Medical University, 246 Xue fu Road, Nangang District, Harbin 150086, Heilongjiang, China, Email: xczhang1973@hrbmu.edu.cn

Accepted Date: Mar 21, 2022 / Published Date: Mar 28, 2022

Abstract

To study the effects of the unfolded protein response (UPR) activation and autophagy inhibition on sorafenib inducing apoptosis of hepatocellular carcinoma (HCC) cells. We activated UPR of the HepG2 cells by dithiothreitol (DTT) and tunicamycin (Tun). 3-methyladenine was used to inhibit autophagy of HepG2 cells. The proliferation, apoptosis and adhesion ability of HCC cells were tested by MTT assay and flow cytometry, respectively. The invasion and migration abilities of cells were detected by Trans well assay. Moreover, we established HCC orthotopic transplantation tumor model of nude mice. The activation of UPR can inhibit the sorafenib-induced apoptosis of HCC cells. Inhibiting autophagy significantly enhanced the sorafenib-induced apoptosis of HCC cells. Activating the UPR can enhance the sorafenib resistance of HCC cells. On the contrary, inhibiting autophagy causes opposite effects.

Citation: Wu J, Zhuang Q, Gao X, Yang X, Qin R, et al. (2022) The Study of the Influence of Unfolded Protein Response and Autophagy on Sorafenib-Induced Tumor Inhibition of Hepatocellular Carcinoma. Cell Mol Biol, 68: 227. Doi: 10.4172/1165-158X.1000227

Copyright: © 2022 Wu J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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