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  • Research Article   
  • Cell Mol Biol 2018, Vol 64(3): 152

UNBS5162 Inhibits U251 Glioma Cells Proliferation and Metastasis by Promoting Apoptosis

Luo Li1*, Pin Guo2, De-Hong Wan2 and Guang-Ping Liu2
1Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, P.R. China
2Department of Neurosurgery, Qingdao Municipal Hospital, Qingdao, Shandong, P.R. China
*Corresponding Author : Luo Li, Department of Neurosurgery, The Qingdao Municipal Hospital, No.5 Donghaizhong Road, Qingdao, Shandong 266071, P.R. China, Tel: +86 532 8291 1201, Email: liluo1122334455@163.com

Received Date: Jul 11, 2018 / Accepted Date: Oct 10, 2018 / Published Date: Nov 10, 2018

Abstract

Naphthalimides have potent anti-cancer activity against human cancer cells. UNBS5162, as a derivative of naphthalimide, is capable of removing primary amine group and reducing toxicity. Recently studies have discovered that UNBS5162 could significantly down-regulate pro-angiogenic chemokines in human prostate tissue, indicating it might have an anti-angiogenic effect. In this work, we intended to explore the functional role of UNBS5162 on glioma cells proliferation, migration and invasion. Cell viability, migration and invasion capabilities of U251 cells were examined by Cell Counting Kit-8 (CCK-8) and transwell assays. The cell apoptosis was tested by Annexin V-FITC/ PI and flow cytometry. Apoptosis-associated proteins and the related-markers of Phosphatidylinositol 3-kinase (PI3K) were examined by western blot analysis. The CCK-8 test suggested that UNBS5162 markedly suppressed the viability of glioma cell line U251. UNBS5162 markedly repressed glioma cell migration and invasion, which was shown from results of transwell assay. Further, UNBS5162 promoted glioma cell apoptosis rate, accompanied by reduced expression level of anti-apoptotic protein Bcl-2 and elevated expression level of pro-apoptotic protein Active Caspase-3 and Bax in U251 cell line administrated with UNBS5162. Additionally, UNBS5162 suppressed the Phosphatidylinositol 3-kinase (PI3K) signaling pathways in U251 cell line. Therefore, the current finding unveils that UNBS5162 can repress glioma cell viability and induce glioma cell apoptosis by regulating the PI3K signaling pathway, suggesting it might be a potentially promising drug target for clinical glioma therapy.

Keywords: UNBS5162; Glioma; Proliferation; Apoptosis

Citation: Li L, Guo P, Wan DH, Liu GP (2018) UNBS5162 Inhibits U251 Glioma Cells Proliferation and Metastasis by Promoting Apoptosis. Cell Mol Biol 64: 152.

Copyright: © 2018 Li L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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