alexa Uncertainty in the Utility of Mismatch Repair Protein Expression measured by Immunohistochemistry in a Multicenter Population-based Study of Epithelial Ovarian Cancer | Abstract
ISSN: 2161-0681

Journal of Clinical & Experimental Pathology
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Uncertainty in the Utility of Mismatch Repair Protein Expression measured by Immunohistochemistry in a Multicenter Population-based Study of Epithelial Ovarian Cancer

Domenico Coppola1,4*, Santo V. Nicosia5, Andrea Doty2, Thomas A Sellers2, Ji-Hyun Lee3, Jimmy Fulp3, Zachary Thompson2,Sanja Galeb2, John McLaughlin6, Steven A Narod7, Joellen Schildkraut6 and Tuya Pal2

1Department of Anatomic Pathology, H. Lee Moffit Cancer Center, Tampa, Florida, USA

2Departments of Cancer Epidemiology, H. Lee Moffitt Cancer Center, Tampa, Florida, USA

3Department of Biostatistics, H. Lee Moffitt Cancer Center, Tampa, Florida, USA

4Department Experimental Therapeutics, H. Lee Moffitt Cancer Center, Tampa, Florida, USA

5Department of Pathology and Cell Biology, University of South Florida, College of Medicine

6Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada

7Centre for Research in Women’s Health, Women’s College Research Institute, Toronto, Canada

*Corresponding Author:
Domenico Coppola, MD
Department of Anatomic Pathology Moffitt Cancer Center
Departments of Oncologic Sciences
University of South Florida 12902 Magnolia Drive Tampa
Florida 33612, USA
E-mail: [email protected]

Received date: June 26, 2012; Accepted date: July 11, 2012; Published date: July 14, 2012

Citation: Coppola D, Nicosia SV, Doty A, Sellers TA, Lee JH, et al. (2012) Uncertainty in the Utility of Mismatch Repair Protein Expression measured by Immunohistochemistry in a Multicenter Population-based Study of Epithelial Ovarian Cancer. J Clin Exp Pathol 2:115. doi:10.4172/2161-0681.1000115

Copyright: © 2012 Coppola D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


immunohistochemistry (IHC) for MMR protein expression. Although the utility of IHC has been demonstrated in
colorectal cancer, its utility in ovarian cancer remains incompletely defined. We sought to evaluate the loss of protein
expression of the MMR proteins (MLH1, MSH2 and MSH6) in three population-based samples of epithelial ovarian
Methods: IHC staining was performed on full section slides or tissue microarray (TMA) slides for MLH1, MSH2,
and MSH6 protein expression in paraffin-embedded tumor samples. Samples were considered to have loss of
expression (LoE) if one or more proteins had an expression score of < 3 (on a scale of 9). Collection of demographic,
clinical and family history information was attempted in all women.
Results: Of 487 participants in whom IHC was completed, 147 were performed through full section slides and
340 were performed through TMA slides. In the overall sample, LoE of one or more of the MMR proteins was observed
in 12.7%. Notably, the proportion of tumors with LoE was significantly higher in TMAs (17.9%) compared to full section
slides (0.7%) (p <0.001). Finally, tumors with loss of MMR protein expression were more likely to be from individuals
with tumors of non-serous histology (p=0.004).
Conclusions: A substantial proportion of epithelial ovarian cancers have a loss of MMR protein expression.
Protein expression results vary significantly by the tissue sampling methodology utilized, raising concerns about the
clinical utility of this test for ovarian tumors.


Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

+1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version