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Validation Study of UPLC Method for Determination of Morphine, Ropivacaandiuml;ne and Ziconotide in Combination for Intrathecal Analgesia | OMICS International| Abstract
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
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  • Research Article   
  • J Anal Bioanal Tech 2016, Vol 7(3): 310
  • DOI: 10.4172/2155-9872.1000310

Validation Study of UPLC Method for Determination of Morphine, Ropivacaïne and Ziconotide in Combination for Intrathecal Analgesia

Rossignol E1,2, Sorrieul J1*, Beaussart H1, Kieffer H1, Folliard C1, Dupoiron D3 and Devys C1
1Institut de Cancérologie de l’Ouest Paul Papin-Pharmacy, , Angers, France
2Institut de Cancérologie de l’Ouest René Gauducheau-Biology, , Saint Herblain, France
3Institut de Cancérologie de l’Ouest Paul Papin-Anesthesia and Pain department, , Angers, France
*Corresponding Author : Sorrieul J, Institut de Cancérologie de l’Ouest Paul, Papin-Pharmacy, Angers, France, Tel: 0241352721, Fax: 0241352866, Email: [email protected]

Received Date: Mar 10, 2016 / Accepted Date: Mar 21, 2016 / Published Date: Mar 28, 2016


Pain is often considered as the most feared symptom amongst individuals living with cancer. It can arrive at any stage during the course of the illness. In 15% to 20% of patients, conventional analgesic therapy either fails to relieve pain or induces adverse effects. Use of analgesic admixture has been recommended by the most recent consensus conferences. Several studies found evidence of synergistic effects of intrathecal analgesic admixtures, most notably these containing ziconotide, morphine and ropivacaïne, administering by a fully implantable pumps. The refills were prepared under a laminar airflow hood under strictly aseptic conditions, by the hospital pharmacist. This group of drugs that commonly used can be at the origin of errors inducing adverse effects in patients. In order to evaluate the accuracy of compounding of intrathecal admixtures, a new analytical method by simple liquid chromatography ultraviolet spectrometry method was developed and validated for the simultaneous quantification of three analgesic drugs (morphine, ropivacaïne and ziconotide). The method was validated according to the recommendation of the US Food and Drug Administration (FDA). The method was linear, between 0.1 to 4 μg/ml for ziconotide, 0.1 to 10 mg/ ml for ropivacaïne and 0.1 to 32 mg/ml for morphine. Forced degradation of ziconotide by acidic conditions allowed formation and detection of degradation products by the analytical method. This method can be considered as a stability indicating method. It is also part of a continuing quality process designed to improve accuracy of preparation.

Keywords: Intrathecal analgesia; Morphine; Ropivacaïne; Ziconotide; UPLC-UV

Citation: Rossignol E, Sorrieul J, Beaussart H, Kieffer H, Folliard C, et al. (2016) Validation Study of UPLC Method for Determination of Morphine, Ropivacaïne and Ziconotide in Combination for Intrathecal Analgesia. J Anal Bioanal Tech 7:310. Doi: 10.4172/2155-9872.1000310

Copyright: © 2016 Rossignol E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Review summary

  1. Ronald
    Posted on Aug 04 2016 at 5:31 pm
    The article describes the study that studies the efficacy of the UPLC method in the process of determination of analgesics such as Morphine, Ropivacaïne and Ziconotide in the formulation suggested by the US Food and Drug Administration (FDA) for the treatment of pain in cancer patients. The article provides significant knowledge regarding the pros and cons of the method and will be used to improve the existing method of analgesic administration in cancer patients.