ISSN: 2168-9652

Biochemistry & Physiology: Open Access
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
  • Review Article   
  • Biochem Physiol 2018, Vol 7(1): 226
  • DOI: 10.4172/2168-9652.1000226

Physiological and Ultrastructural Alterations in the Crayfish Procambarus clarkii Treated with Spinosad (Bacterial Derived Insecticide)

Abd El-Atti MS* and Radwa M Saied
Department of Zoology, Faculty of Science, Zagazig University, Zagazig, Egypt
*Corresponding Author : Abd El-Atti MS, Department of Zoology, Faculty of Science, Zagazig University, Zagazig, Egypt, Tel: + 00201142757608, Email: mahmoudatti1@yahoo.com

Received Date: Nov 07, 2017 / Accepted Date: Jan 12, 2018 / Published Date: Jan 20, 2018

Abstract

Physiological and ultrastructural investigations have been carried out on Procambarus clarkii exposed to sublethal concentrations of Spinosad insecticide. The study showed that the highest mortality percentage was 88% for males and 70% for female occurred at 64 × 10³ ppm Spinosad. The lethal toxicity (LC50) was 24.1 g/l and 29.8 g/l for males and females respectively. Exposure to 1/2 LC50 (12.07 g/l). Spinosad for 7 days caused many physiological disorders including decrease the haemolymph glucose levels and elevated uric acid levels.12.07 g/l Spinosad resulted in a significant decrease in the total proteins of haemolymph, hepatopancreas and muscles in both males and females. The total cholesterol and triglycerides levels were significantly increased in both haemolymph and hepatopancreas and decreased in muscles of males. On the other hand, cholesterol and triglycerides levels were significantly decreased in haemolymph, hepatopancreas and muscles of females. TEM examinations of male hepatopancreas exposed to 12.07 g/l Spinosad for 7 days revealed ruptured microvilli of absorptive cells, deformed mitochondria, destructed rough endoplasmic reticulum, vacuolated cytoplasm, pyknotic nuclei and appearance of vesicles containing small dark granules.

Keywords: Physiological; Ultrastructure alterations; P. clarkii spinosad

Citation: El-Atti MSA, Saied RM (2018) Physiological and Ultrastructural Alterationsin the Crayfish Procambarus clarkii Treated with Spinosad (Bacterial DerivedInsecticide). Biochem Physiol 7:226. Doi: 10.4172/2168-9652.1000226

Copyright: © 2018 El-Atti MSA, et al. This is an open-access article distributedunder the terms of the Creative Commons Attribution License, which permitsunrestricted use, distribution, and reproduction in any medium, provided the originalauthor and source are credited.

Top