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Dissecting Signaling Pathways by the Use of Genetically-Encoded Biosensors: Dynamic Matters
Among biosensors, genetically-encoded FRET biosensors raised hope to dissect signaling pathways by monitoring enzymatic activities or second messengers levels in living cells and even in living and developing organisms. FRET (Froster resonance Energy Transfer) is a radiationless energy transfer from donor to acceptor fluorophore.. Read More
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Biosensors for Indirect Monitoring of Foodborne Bacteria
Microbiological safety assessment in food samples is often a cumbersome and time consuming task that has to be done in a regular basis in most food manufacturing facilities. Standard microbiological techniques for evaluating critical levels of bacteria take several days to yield results, thus the food products have to be withhel.. Read More
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A Point of Care Real Time PCR Platform Based on Silicon Technology
The miniaturization of a real time PCR amplification systems are key to genetic point-of-care (POC) diagnostics to offer sample-in answer-out analysis. Actually, miniaturization typically enables shorter analysis times, reduces reagent consumption, minimizes risk of sample contamination, and often enhances assay performance. Her.. Read More
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A rapid overview of the Three Phase Partitioning (TPP) system as an efficient non-chromatographic tool is given. This elegant non-chromatographic tool is able to purify numerous proteins and especially proteases in a one step protocol. TPP is able to do not only purify proteins, but also concentrate them into one of the phases a.. Read More
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Pesticides Detection Using Acetylcholinesterase Nanobiosensor
Pesticides are chemical substances used for crop improvement and these substances when degraded leave behind certain toxic residues which can be detected using nano biosensors which are relatively inexpensive, does not require sample pre-treatment and provides sensitive screening. The application of nanobiosensors for detection .. Read More
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Universal Bioanalyte Signal Amplification for Electrochemical Biosensor
A rapid, simple, and inexpensive electrochemical biosensor with guanine-conjugated microbead tags can achieve the detection limits of PCR and culturing. Guanine is a redox species that functions as an electrochemical tag for bioanalyte detection. Microbeads conjugated with guanine-rich oligonucleotides deliver millions of electr.. Read More
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Analgesic and CNS Depressant Effect of the Crude Ethanolic Extract of the Operculina turpethum
The Operculina turpethum (Fam. Convolvulaceae) is a traditional medicinal plant for the treatment of anthelmintic, expectorant, antipyretic, anti-inflammatory and purgative. The work reached the acute toxicity of Operculina turpethum and its action on the ethanolic extract of the central nervous system (CNS) because on data in t.. Read More
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Biosensors: Recent Advancements in Tissue Engineering and Cancer Diagnosis
Biosensors are the devices, which integrate the biological recognition element and a small transduction unit for the purpose of detecting various analytes. Biosensors are being developed for various applications such as quality control of food, agriculture, medical and pharmaceutical field, clinical assay and disease diagnostics.. Read More
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Blocking Non-Specific Binding for Phage-Based Magnetoelastic Biosensors
The magnetoelastic (ME) biosensors are used to detect pathogen in fresh juice or milk by solenoid coil, and also developed for real-time, direct pathogen detection on food surfaces by surface-scanning coil. This paper presents blocking effect of different reagents on non-specific binding for detecting Salmonella typhimurium in a.. Read More
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Recent Update of Nanobiosensors Using Olfactory Sensing Elements and Nanomaterials
Various biomaterials such as antibody, aptamer, enzyme, DNA and sensory receptor can be used for recognizing ligands by nanobiosensor. Especially, olfactory sensing elements with olfactory receptor are most promising one to develop the nanobiosensor with high performance because they can discriminate target molecules with high s.. Read More
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One-Step Electrodeposition of Gold-Graphene Nanocomposite for Construction of Cholesterol Biosensor
The determination of cholesterol is increasingly important for the fact that high serum cholesterol level was related with many diseases. In this work, a new cholesterol biosensor was developed based on the gold-graphene nanocomposite material prepared by one-step electrodeposition. The nanomaterial formed on a glassy carbon ele.. Read More
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Whispering Gallery Microcavity Nanosensor with In-Line Interferometer
Here we demonstrate a simple but robust interferometry scheme that connects an ultra-high quality factor whispering gallery micro cavity to a FC/PC connectorized single mode fibre. With this scheme, single Polystyrene beads with a radius as small as 25 nm can be detected at a signal-to-noise ratio of around 3... Read More
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A Method for Screening Sensors of Calcium Signaling in Rice
Although rice is an important staple food crop of nearly half of the world population, the discoveries in calcium ion signal were mainly focused on the model plant Arabidopsis and researches in rice are very superficial and no calcium ion sensors have been found yet. Recently, the recombinant aequorin was introduced into rice, a.. Read More
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Emerging CMOS Bio-Sensors: A Paradigm Shift
It is well-established fact that the CMOS foundries, with several trillion dollars investment, are efficient mass-production platforms that lowered the cost of microelectronic devices, low enough to make most of consumer electronic products affordable to the endusers. As to their scale of integration, today’s foundry proce.. Read More
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The term biosensor appeared in the 1960s to define the use of bio electrodes involving enzyme electrodes that presented and bio catalytic activity. In 1962, brought the very rudimentary model of biosensors forward with the early invention of an oxygen electrode in which the electrochemical detection of oxygen or hydrogen peroxid.. Read More
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We have fabricated a fiber optic SPR biosensor for the detection of vitellogenin (Vg), an endocrine disruption biomarker in aquatic environments. The sensor was fabricated by immobilizing anti-Vg antibodies on the sensor surface. Control experiments performed on another similar protein, fetuin and on a sensing probe without the .. Read More
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In data acquisition and digital instrumentation fields, it is essential to understand the learning and recognition to acquire data and information of objects to be studied. In recent years, engineering modelling and simulation contribute greatly to the understanding of intelligent learning and recognition problems. The ability t.. Read More
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A simple, specific and rapid identification system against Bacillus anthracis spores was developed using specific capture peptides conjugated with a bead-based biosensor, which was tightly and specifically bound onto the spore surface of B. anthracis. We successfully detected and separated B. anthracis spores from the spores of .. Read More
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A New Chemiluminescent Enzyme Immunoassay for Plasma Tissue Factor Detection
High concentrations of tissue factor (TF) in the plasma may have a major role in the elevated risk of thrombosis in patients with malignant diseases. The quantification of plasma TF concentrations in cancer patients may enable accurate assessments of the risk of thrombosis during the process of cancer progression. Whether the pl.. Read More
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Survey of Popular Networks Used for Biosensors
Miniaturized electronic components, improvements in Micro-Electro-Mechanical Systems (MEMS), have made realization of biosensors possible. Providing interconnectivity to these devices has made even more applications possible. Today biosensors are being used in many applications ranging from detectıon of biological a.. Read More
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