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Research Article Open Access
The miniaturization of a real time PCR amplification systems are key to genetic point-of-care (POC) diagnostics to offer sample-in answer-out analysis. Actually, miniaturization typically enables shorter analysis times, reduces reagent consumption, minimizes risk of sample contamination, and often enhances assay performance. Here we present the genetic real time PCR PoC system based on silicon microchip able to address the quantitative and qualitative identification of multiple analytes of specific nucleic acids (DNA, RNA) sequences. Thanks to the combination of integrated silicon temperature sensors and heaters, the specific chip design architecture and the smart detection software, an improvement of sensitivity is achieved respect to the commercial tools.
Real time PCR, Microchip, DNA, Biochemical Engineering, Biochemical Methods