Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
Reach Us +44 3308186230


Journal of Pregnancy and Child Health - Prevalence of Iron Deficiency Anemia in Pregnant Women of District Mardan, Pakistan
ISSN: 2376-127X

Journal of Pregnancy and Child Health
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Prevalence of Iron Deficiency Anemia in Pregnant Women of District Mardan, Pakistan

Sulaiman Shams1*, Zarmina Ahmad1,2 and Abdul Wadood1
1Department of Biochemistry, Abdul Wali Khan University Mardan, Pakistan
2Department of Biochemistry, Bacha Khan Medical College Mardan, Pakistan
*Corresponding Author: Sulaiman Shams, Department of Biochemistry, Abdul Wali Khan University Mardan, Khyber Pakhtunkhwa, Pakistan, Tel: +923339202865, Email:

Received: 16-Oct-2017 / Accepted Date: 01-Nov-2017 / Published Date: 08-Nov-2017 DOI: 10.4172/2376-127X.1000356


Background: Iron depletion is the most common nutrient deficiency worldwide and is the leading cause of anemia. Anaemia in pregnancy is a common clinical problem contributing to increased maternal and foetal morbidity. Previously few studies have highlighted this problem in developing country like Pakistan but the situation needs further exploration in pregnant women of less developed areas. The current study aimed to estimate the prevalence of iron deficiency anemia (IDA) among pregnant women of district Mardan, Khyber Pakhtunkhwa (KP), Pakistan.

Methods: The study consisted of 300 pregnant women in the age group (18-40) years. Blood samples were collected from each pregnant woman and a questionnaire was completed at the time of blood collection. Hematological and biochemical profiles were determined and the collected data was analyzed using SPSS.

Results: In the current study the overall prevalence rate of iron deficiency anemia in pregnant women was (76.7%). Iron deficiency anemia was most prevalent in the second trimester (45.7%) as compared to first (16.1%) and third (38.2%) trimester pregnancy. Of the anemic cases, 42.6% have low iron stores and 43.5% have serum ferritin in a range of 12-30 ng/ml. In 60% of cases CRP was raised and 72.2% were multiparous. Of the study population, 65% were uneducated and 87% belonged to lower middle class.

Conclusion: This study concluded that anemia is highly prevalent among the antenatal women of this area and iron deficiency considered to the culprit behind this disease. Multiparty, low socio-economic status and low education are the contributory factors of iron deficiency anemia. There is a great need for further health education promotional programs to improve heath of pregnant women.

Keywords: Pregnancy; Haematological and biochemical profiles; Mardan; Anemia; Iron deficiency


Anaemia from Greek Anaimia, “meaning without blood” is as a condition in which either the number of red blood cells (RBCs) decreased or their oxygen-carrying capacity reduce to meet the body normal physiologic functions. Anemia is especially common in women of reproductive age and particularly during pregnancy. Anemia is a well-studied and well known risk factor in pregnancy for both mother and fetus life. Anaemia is associated with increased pre-term labour (28.2%), pre-eclampsia (31.2%) and maternal sepsis [1,2]. Three types of anemia during pregnancy are severe anemia when hemoglobin concentration is less than 7.0 g/dL, moderate when hemoglobin falls between 7.0-9.9 g/dL and mild anemia when hemoglobin level range from 10.0 to 11.0 g/dL [3,4]. Anemia has a variety of converging contributing factors including nutritional, genetic, frequent labour, multiparity, abortions and infectious disease, however, iron deficiency is the cause of 75% of anemia cases [1,5,6]. The major causes of iron deficiency include insufficient intake of iron-rich foods and poor bioavailability of consumed iron in relation to the need during pregnancy [7]. The demand for iron increases about six to seven times from early pregnancy to the late pregnancy [8].

Iron deficiency anemia (IDA) is a well-reported problem during pregnancy in both developed and developing countries including Pakistan. In the world the number of anaemic people around 1.62 billion among which 56 million are pregnant women [5]. Prevalence of iron deficiency anemia among women in developing countries was calculated from 40% to 88%. In Pakistan the prevalence of anemia among pregnant women living in urban areas was reported from 29% to 50% [9]. Some studies have shown that the frequency of Iron deficiency anemia varies in the pregnant women of Karachi (64%), Lahore (73%) and Multan (76%) [10-12]. Although the differences among the reported prevalence rates in the studies might be due to variations in the main characteristics of their target population, methodological differences such as laboratory tests. But Despite its known effect on the population, very limited data is available on the biochemical deficiency of micronutrient like iron in pregnant women of the studied region of Khyber Pakhtunkhwa (KP). Identifying the prevalence of anaemia and determining its causes in individuals of high-risk groups, such as in pregnant women is a dire need of the day particularly in developing countries like Pakistan, where the social conditions pose serious challenges to women. Therefore, this study was carried out to determine the prevalence of anemia and true iron deficiency in the pregnant women of district Mardan and to compare it with previously done studies.

Materials and Methods

Study design, study population and sampling size

This was a descriptive, cross sectional type of study. The study population comprised 300 women aged 18-40 years, at various stages of pregnancy belonging to different socioeconomic backgrounds. A total of 300 apparently healthy pregnant women were studied attending the out patient department of Gynae and Obstetric Unit of Mardan Medical Complex.

Inclusion and exclusion criteria

Healthy pregnant women, aged 18-40 years at different stages of pregnancy belonging to district Mardan were selected, whose Haemoglobin level were below 11 g/dl. While excluded pregnant women with any acute illnesses, gestational diabetes mellitus, hypertension, obesity, women with other chronic diseases, women over age 40 and women with a history of blood loss/blood transfusion in the present pregnancy.

Ethical consideration

This study was approved by the ethics committee of the Abdul Wali Khan University Mardan KP and performed in accordance with the principles of Committee. To ensure their voluntary participation, inform consent was obtained from all the participants.

Questionnaire interview

A questionnaire based interview was done from all the participants. All interviews were conducted face to face by the researcher’s their self. During the survey the researcher explained a question that was not clear. The questionnaire included questions on socio-demographic data (age, education and family income/month), medical history (previous pregnancy, complications in previous pregnancy), clinical data (complications during this pregnancy, any blood loss and treatment received, (if any) food and drink intake.

Specimen collection and determination of hemoglobin

Blood samples were collected from 300 pregnant women who were at various stages of pregnancy. The blood samples were collected in Ethylenediaminetetraacetic acid (EDTA) tubes. Hemoglobin determinations of 300 females were done. Hemoglobin was measured by hematology analyzer (BC 3000 Plus Auto hematology analyzer, MINDRAY: Bio-medical Electronics company Ltd., Shenzhen China) at the pathology and hematology laboratory Bacha Khan medical college Mardan. Blood samples were withdrawn and collected in EDTA tubes, labeled and processed in the laboratory. The samples were then inserted into the analyzer and automated measurement was initiated. Participant’s results were recorded in laboratory information system. The results compared with the previous data and any variations such as abnormal drop in hemoglobin, flagged. The procedure was repeated three times.

Measurements of blood iron stores

The blood iron stores were measured by estimation of serum ferritin in those anemic females whose hemoglobin level was less than 11 g/dl. Blood samples were collected in EDTA tubes and stored at 2-8°C for 3-5 days. Estimation of serum ferritin was done collectively of 5-10 samples. Serum ferritin estimation was done by using Elecsys and COBAS e (Trade mark of Rosche) immunoassay analyzer. The results then collected. A few cases were seen to have raised serum ferritin so in those cases further estimation of CRP was done.

Estimation of C-reactive protein (CRP)

CRP was estimated by latex agglutination method. The CRP latex is a slide agglutination test for the qualitative and semi-quantitative detection of C-Reactive protein in human serum. The kit was used of ANTECH Diagnostics. Add 50 ul of the sample and one drop each of positive (Human serum with CRP concentration >20 mg/L Sodium azide 0.95 g/L) and negative controls (Animal serum sodium azide 0.95 g/L) into separate circles on the test slide. The CRP latex reagent was swirled gently before using. Then 50 μl of the reagent was added to the samples. The drops mixed and spread over the entire surface with a stirrer. Different stirrers were used for different samples. The slide was put on a mechanical rotator at 80-100 rpm for 2 min. Then the slide examined macroscopically for the presence or absence of any visible agglutination. The presence of agglutination showed a CRP concentration of equal to or greater than 6 mg/L. CRP concentrations were determined in triplicate and the mean was used for analysis.

Care manager

Five care managers were working in the office and Clinic of Zarmina Ahmad at Bacha Khan Medical College And in office of Dr. Sulaiman Shams in Biochemistry Department AWKUM. The central aim of the care managers was to empower pregnant females living with iron deficiency to take a more active role in their health.

Statistical analysis of the data

All data are presented as mean ± SD. Significant differences were determined by using ANOVA in SPSS 16.0. P


From different localities of district Mardan, 300 pregnant women were selected, for the purpose of this study. Detailed medical and obstetric history was recorded. Out of 300 women, 230 fulfilled the criteria and further studies were carried out.

Socio-demographic data of the study population

The study population comprised of 300 pregnant women, aged 18-40 years. Table 1 summarizes their socio-demographic details. It showed that out of total, 60% women were in age group 30-40 years while 40% were in age range 18-29 years. Almost 65% were not educated (never attended school or have education less than class 5) and 35% had some educational background (have completed education up to class 5 and above). Most of these pregnant women belonged to lower (annual house hold income US$<500) and middle class (annual house hold income US$500-1000) that comprised 87% of the whole group and 13% belonged to upper class (annual house hold income U$>1000. Regarding their residence, 70% of the females came from rural areas while 30% were living in the urban areas of district Mardan (Table 1).

Characteristics Range/Group   Number         %
  Age Group 18-29 years         92       40%
30-40 years         138       60%
Education Educated         80       35%
Non educated         150       65%
Socio-economic status Lower class         92       40%
Middle class         108       47%
Upper class         30       13%
Residence Urban Areas         69       30%
Rural  areas         161       70%

Table 1: Socio-demographic distribution of studies population.

Status of pregnancy in iron deficient women

Out of the total, almost half (45.7%) of the pregnant women were in their 2nd trimester of pregnancy, followed by 3rd trimester (38.2%) and the rest of females (16.1%) were in their 1st trimester. The current study also reflected that most of the women (72.2%) were multi gravida, while 19.1% were having their first pregnancy and 8.7% were grand multiparous women as shown in Table 2.

Status No. of Participants %
Pregnancy Status 1st Trimester 37 16.1%
2nd Trimester 105 45.7%
3rd Trimester 88 38.2%
Parity  Status Primigravida 44 19.1%
  Multigravida 166 72.2%
Grand multi 20 8.7%
Total participants   230  

Table 2: Status of pregnancy in iron deficient women.

Prevalence of anemia in study population

Estimation of hemoglobin level of 300 women was done. Out of total studies population, 230 women have hemoglobin level less than 11 g/dl. The current study showed the prevalence of anemia (defined by the World Health Organization as hemoglobin <11.0 g/dL) in these subjects was 76.7% (Table 3).

      Hb level No. of Women Percentage
     Patients    <11 g/dl       230    76.7%
     Normal    >11 g/dl       70    23.3%
     Total                                     300    100%

Table 3: Prevalence of anaemia in study population.

Prevalence of anemia based on severity of anemia

Of the total anemic cases, 68.3% had mild anemia and 29.1% had moderate anemia and 2.6% were severely anemic as shown in Table 4. Grading of anemia is done on WHO classification of anemia (Table 4).

Group Severity of Anemia Haemoglobin Levels (g/dL)    No. of patients          Patients (%)
  1  Mild  10-10.9         157          68.3%
  2  Moderate  7-10          67          29.1%
  3   Severe  Less than 7           6          2.6%

Table 4: Prevalence of anemia based on severity of anemia.

Serum ferritin level in study population

To determine the actual prevalence of iron deficiency anemia in the study population, further analysis was carried out of those participants who had hemoglobin concentration of less than 11 g/dl. Category 1 (<12 ng/ml), requires active intervention. Category 2 (12-30 ng/ ml) level which still signifies low iron stores. Category 3 (31-100 ng/ ml) indicates a normal level. As serum ferritin is also an acute phase reactant so its level rises in response to infections. Category 4 showed levels >300 ng/ml. The current study showed as in Table 5 that out of total 230 participants, 42.6% had Serum ferritin level below12 ng/ ml. while 43.5% had serum ferritin level in the range of 12-30 ng/ml which is a range that still signifies the low iron stores in almost half the population. 11.7% have S. ferritin level in 31-300 ng/ml rang and the rest of 2.2% of the participants have a high level of S. Ferritin, i.e., >300 ng/ml (Table 5).

Category Serum Ferritin Level (ng/mL)         No. of Participants     Percentage
Category 1 <12 ng/mL            98                 42.6%
Category 2 12-30 ng/ml            100                  43.5%
Category 3 31-300 ng/ml            27                   11.7%
Category4 >300 ng/ml            5                   2.2%

Table 5: Serum ferritin level in study population.

Co- relation of anemia with low S. ferritin

Table 6 determines the Comparison of anemia with positive serum ferritin in study population. Out of severely anemic cases, 83.3% had low S. ferritin (Table 6).

Type of anemia & No. of Patients No. & % of participants S. ferritin <12 ng/ml    No. & % of participants S. ferritin 12-30 ng/ml No. & % of Participants S. ferritin 30-300 ng/ml
Mild Anemia
      68 (43.3%)           30 (19.1%)             59 (37.6%)
Moderate anemia
      22 (32.8%)       26 (38.8%)       19 (28.4%)
Severe anemia
       5 (83.3%)         1 (16.7%)         Nil

Table 6: Comparison of anemia with S. ferritin in study population.

CRP relative to raised S. ferritin

We performed CRP on those 5 women with raised positive S. Ferritin, i.e., >300 ng/ml. The result showed that 3 out of these 5 women had raised CRP as shown in Table 7.

No. of patients Hb in g/dl S. ferritin in ng/ml   CRP levels
           1        10.2             460      Raised
           2        10.8             320      Normal
           3        9.8             304      Normal
           4        10.0             502      Raised
           5        10.1             330      Raised

Table 7: Comparison of raised S. ferritin with CRP levels.


The single most prevalent micro nutrient i.e., iron deficiency coupled with anemia in a pregnant woman has serious health threats. The prevalence of IDA in developing countries including Pakistan remains high. Pakistan, with its vital geopolitical importance, is still a long way off from its goal to overcome IDA among vulnerable population groups. According to a report of national health survey of Pakistan, 50-60% of expectant mothers are affected by IDA [13]. In an effort to combat this seemingly benign problem, we first aimed to get the prevalence of anemia generally in the pregnant population of district Mardan. This study showed the prevalence of anemia in these subjects to be quite high (76.7%) as sown in Table 3, of these 68.3% had mild anemia and 29.1% had moderate anemia. 2.6% were severely anemic. Secondly the results were compared with other studies done in Pakistan and especially in KP. It showed that in a study done in district Karak, the percentage of anemia in pregnant women was 67.6%, while a study done in Kohat showed the prevalence of anemia to be 66.6% [14,15]. Studies done in Peshawar showed the prevalence to be 53.0% (Table 4) [16].

The anemia association with education in the present study was that 65% were un-educated (never Attend school) and 35% had some educational background. The study demonstrated that less education was associated with high prevalence of anemia. This is similar to studies carried out in Korangi Karachi and district Karak that showed high incidence of anemia in illiterate pregnant females (55.6% and 88.0%, respectively) [10,14]. From the above information it is evident that lower the level of education in women, the more probability of anemia during. Most of these pregnant women in the current study belonged to lower middle class that comprised 87% of the whole group 13% belonged to upper class. This is similar to the study done in District Karak where 80% of anemic pregnant females belonged to lower class [14]. Regarding their residence in the present study, 70% of the females came from rural areas while 30% were living in the urban areas of district Mardan. The Table 2, determine that the incidence of anemia was high in the second trimester (45.7%) and the rest of females were either in their 1st trimester (16.1%) or 3rd trimester (38.2%). The fact that every 2nd pregnant woman is anemic requires some serious measures to be taken towards improvement. This study also reflected that most of the women (72.2%) were multi gravida, while 19.1% were having their first pregnancy. 8.7% were grand multi parous women as shown in Table 2. This is in conformity with the results of other similar studies which also signify that multiparity and short birth spacing lead to anemia in women (Tables 1 and 2) [17].

Another focus of the current study was to know the prevalence of actual iron deficiency in these anemic patients. Serum ferritin levels were estimated in the blood samples of these anemic subjects. While interpreting the levels of serum ferritin, the cases were divided into four categories as shown in Table 5. This study showed that in almost half the population of pregnant females (42.6%) S. ferritin level was below 12 ng/ml establishing iron deficiency as the main cause of anemia in pregnant females as shown in Table 5. In the current study, 43.5% have serum ferritin in the range of 12-30 ng/ml which is a range that still signifies low iron stores. Only 11.7% had a normal value of S. Ferritin, i.e., 31-300 ng/ml. Although women with acute or chronic infections were excluded from the study to get a clear picture regarding levels of iron stores, still in 2.2% cases the value of serum ferritin was >300 ng/ ml as shown in Table 5. For better interpretation of the results, the cases were tested for CRP levels. It turned out that 60% of cases, i.e., 3 out of 5 had raised CRP as shown in Table 7. An important strength of the current study is that the study population was homogeneous in terms of race. Determination of hemoglobin with the help of auto analyzer done was performed during the study for the first time in this region. This is the first study that examines the of anemia in the population of Pashtuns women during pregnancy using serum ferritin in addition to CRP to account for infection. The rate of low birth weight babies was high in mothers who were anemic in their third trimester. Preterm deliveries occurred highly in mothers who were anemic in their second and third trimesters [18]. Therefore, the government needs to take action to improve the quality of education and socioeconomic status of females and to increase the number of health care providers.


It can be concluded from this study that anemia due to iron deficiency is highly prevalent in district Mardan. The results of the current study were found in conformity with the work of other researchers in our country. It was observed that the etiology of iron deficiency remains the same over the decades. Multiparity, short birth spacing, poor socio economic status, lack of education were responsible for high prevalence of IDA. Besides this, it was observed that majority of women attended clinic during the 2nd trimester of pregnancy. So they had lack of iron intake at the time of conception. Non-compliance of iron supplementation was also observed to be an important contributory factor. Almost all the South Asian countries including Pakistan has national level anemia control programs but this problem still persists. This study will provide a base upon which strategies against the eradication of IDA will be made. Intervention only with iron and folic acid supplements is not adequate to combat this problem but this issue requires a multi-faceted approach. Besides regular screening of hematological parameters during pregnancy, nutritional education and counseling should as a part of anemia eradication plan. Researchers should concentrate on preventive supplements and food fortification approaches. A detailed health data could be acquired such as parity, menstrual characteristics, infections, previous iron or blood transfusions, etc. The Government needs to take solid steps to improve the quality of education and socioeconomic status of females, increase the number of health care providers and intensify public education. Health behavior’s need to be changed and adherence to the prescribed programs by the government is needed. Providing long term iron supplementation and dietary modification starting from adolescence may improve the hemoglobin levels and later on prevent anemia in pregnancy.


We thank all the pregnant women of district Mardan who participate in this study. Sulaiman Shams and Zarmina Ahmad participated in all steps of the study from its design to write up. Dr. Sulaiman Shams, Zarmina Ahmad and Dr. Abdul Wadood critical review the manuscript and wrote the manuscript and performed statistical analysis.


  1. Sharma JB (2003) Nutritional anaemia during pregnancy in non-industrialized countries. In: Studd J (Edtr), Progress in obstetrics and gynecology. New Delhi: Churchill Livingstone, pp: 103-122.
  2. Buzyan LO (2015) Mild anaemia as a protective factor against pregnancy loss. Int J Risk Saf Med 27: 7-8.
  3. Salhan S, Tripathi V, Singh R, Gaikwad HS (2012) Evaluation of hematological parameters in partial exchange and packed cell transfusion in treatment of severe anaemia in pregnancy. Anaemia.
  4. Esmat B, Mohammad R, Behnam S (2010) Prevalence of iron deficiency anaemia among Iranian pregnant women, a systematic review and meta-analysis. J Reprod Infertil 11: 17-24.
  5. Balarajan Y, Ramakrishnan U, Ozaltin E, Shankar AH, Subramanian SV (2011) Anaemia in low-income and middle-income countries. Lancet 378: 2123-2135.
  6. Masukume G, Khashan AS, Kenny LC, Baker PN, Nelson G (2015) Risk factors and birth outcomes of anaemia in early pregnancy in a nulliparous cohort. PLoS ONE 10: 1-15.
  7. De-Benoist B, McLean E, Egli I, Cogswell M (2008) Worldwide prevalence of anaemia 1993-2005: WHO global database on anaemia. Geneva: World Health Organization.
  8. Christensen RD, Ohls RK (2004) Anaemia’s unique to pregnancy and the perinatal period. Wintrobe’s clinical hematology, USA. Lippincott Williams and Wilkins. 11: 1467-1486.
  9. Lone FW, Qureshi RN, Emmanuel F (2004) Maternal anaemia and its impact on perinatal outcome in a tertiary care hospital in Pakistan. East Mediterr Health J 10: 801-807.
  10. Karim SA, Khurshid M, Memon AM, Jafarey SN (1994) Anaemia in pregnancy-its cause in the underprivileged class of Karachi. J Pak Med Assoc 44: 90-92.
  11. Sohail R, Zainab S, Zaman F (2004) Prevalence of anaemia in obstetrical population. Ann King Edward Med Coll 10: 146-148.
  12. Awan MM, Akbar MA, Khan MI (2004) A study of anaemia in pregnant women of railway colony, Multan. Pak J Med Res 43: 11-14.
  13. Mohyddin M (1995) National health survey of health profile of peoples of Islamabad, Pakistan. Pak Medical Research Council and Federal Bureau of statistics, pp: 27-44.
  14. Irfan U, Muhammad Z, Muhammad IK, Mudassir S (2013) Prevalence of anaemia in pregnant women in district Karak, Khyber Pakhtunkhwa, Pakistan. Int J Biosci 3: 77-83.
  15. Riaz U, Ayaz S, Khader JA, Abdelslam NM, Anwar M (2013) Prevalence and detection of anaemia (Iron Deficiency) in women population in Kohat Khyber Pakhtunkhwa, Pakistan. Life Sci J 10: 812-815.
  16. Paracha PI, Hameed A, Simon J, Jamil A, Nawab G (1997) Prevalence of anaemia in semi-urban areas of Peshawar, Pakistan: A challenge for health professionals and policy makers. J Pak Med Assoc 47: 49-53.
  17. Woldemariam G, Timotiows G (2002) Determinants of nutritional status of women and children in Ethiopia, Calverton, Maryland, USA, pp: 49-57.
  18. Kumar KJ, Asha N, Murthy DS, Sujatha MS, Manjunath VG (2013) Maternal anaemia in various trimesters and its effect on newborn weight and maturity: An observational study. Int J Prev Med 4: 193-199.

Citation: Shams S, Ahmad Z, Wadood A (2017) Prevalence of Iron Deficiency Anemia in Pregnant Women of District Mardan, Pakistan. J Preg Child Health 4: 356. DOI: 10.4172/2376-127X.1000356

Copyright: ©2017 Shams S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.