alexa A Deletion of Signal Peptide in Recombinant Murine Gamma-herpesvirus 68 M3 Protein Enhances its Binding Affinity to CCL5 Chemokine and Increases its Yield from Insect Cells| Abstract
ISSN: 2332-0877

Journal of Infectious Diseases & Therapy
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
  • Research Article   
  • J Infect Dis Ther 2017, Vol 5(6): 347
  • DOI: 10.4172/2332-0877.1000347

A Deletion of Signal Peptide in Recombinant Murine Gamma-herpesvirus 68 M3 Protein Enhances its Binding Affinity to CCL5 Chemokine and Increases its Yield from Insect Cells

Radka Šebová1, Simona Lenhartová2, Iveta Štibrániová1, Ivana Nemčovičová1, Petra Belvončíková1 and Marcela KĂşdelová1*
1Department of Viral Immunology, , Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia
2Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia
*Corresponding Author : Marcela KĂşdelová, Department of Viral Immunology, Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, 00421 02 59302434, Slovakia, Fax: 00421 02 54774284, Email: [email protected]

Received Date: Dec 20, 2017 / Accepted Date: Dec 26, 2017 / Published Date: Dec 29, 2017

Abstract

Objective: The M3 protein encoded by murine gamma-herpesvirus 68 (MHV-68) was the first secreted protein identified in herpesvirus. This protein is unique in its ability to bind a broad-spectrum of chemokines from all four subfamilies, thus it has been proposed to be a potential gene therapy candidate for controlling the overactive inflammatory responses of some human inflammatory diseases.

Methods: We prepared MHV-68 M3del protein with a deletion of its signal peptide (M3del) and full-length MHV-68 M3 protein (M3) using a baculovirus-mediated insect cell expression system and confirmed their specificity by Western blot using newly prepared mouse monoclonal anti-M3 antibody 1/27. Binding affinity of both proteins to human CCL5 and CXCL8 chemokine was examined by ELISA.

Results: M3del and M3 displayed affinity to both chemokines tested. M3 del concentration sufficient to bind 25% of CCL5 was two times smaller than that of M3 (IC25=3.5 nmol/l vs. 6.1 nmol/l). In contrary, the values of IC25 for CXCL8 for M3del and M3 were comparable (IC25=13.8 nmol/l vs. 13.3 nmol/l. We have found that the absence of signal peptide strongly affects the yield of recombinant M3 protein. The yields of M3del and M3 were in an unbalanced ratio of 67 to 1.

Conclusions: The results suggest that the absence of signal peptide in recombinant MHV-68 M3 protein allows increased binding activity of the protein to CCL5 but not to CXCL8, and even a very large increase in its yield from insect cells.

Keywords: Murine gamma-herpesvirus; M3 protein; Chemokines; Signal peptide; Insect cells

Citation: Šebová R, Lenhartová S, Štibrániová I, Nemčovičová I, Belvončíková P, et al. (2017) A Deletion of Signal Peptide in Recombinant Murine Gamma-herpesvirus 68 M3 Protein Enhances its Binding Affinity to CCL5 Chemokine and Increases its Yield from Insect Cells. J Infect Dis Ther 5: 347. Doi: 10.4172/2332-0877.1000347

Copyright: ©2017 Šebová R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Select your language of interest to view the total content in your interested language

Post Your Comment Citation
Share This Article
Relevant Topics
Article Usage
  • Total views: 885
  • [From(publication date): 0-2017 - Mar 26, 2019]
  • Breakdown by view type
  • HTML page views: 847
  • PDF downloads: 38
Leave Your Message 24x7
Top