alexa A Standard Addition Method Utilizing an Endogenous Substance as an In-ternal Standard for Quantitating Arabinofuranosylguanosine 5’ -Triphos- phate in Human Peripheral Blood Mononuclear Cells by Lc-Ms/Ms | Abstract
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

A Standard Addition Method Utilizing an Endogenous Substance as an In-ternal Standard for Quantitating Arabinofuranosylguanosine 5’ -Triphos- phate in Human Peripheral Blood Mononuclear Cells by Lc-Ms/Ms

Yoshiyuki Minamide1*, Harue Igarashi2, Akira Wakamatsu2, Shinobu Kudoh1

1Pharmaceutical and Life Science Division, Shimadzu Techno-Research, Inc., Kyoto, Japan

2Clinical Pharmacology Department, GlaxoSmithKline K.K., Tokyo, Japan

*Corresponding Author:
Yoshiyuki Minamide, Ph.D.
2-13 Nishinokyo-Sanjyo Boucho, Nakagyo-ku
Kyoto 604-8435, Japan
Tel: +81-75-811-3181
Fax: +81-75-821-7837
E-mail: [email protected]

Received date: October 28, 2011; Accepted date: December 06, 2011; Published date: December 08, 2011

Citation: Minamide Y, Igarashi H, Wakamatsu A, Kudoh S (2011) A Standard Addition Method Utilizing an Endogenous Substance as an Internal Standard for Quantitating Arabinofuranosylguanosine 5’-Triphosphate in Human Peripheral Blood Mononuclear Cells by Lc-Ms/Ms. J Anal Bioanal Techniques S5:002. doi: 10.4172/2155-9872.S5-002

Copyright: © 2011 Minamide Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A highly sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for quantitation of arabinofuranosylguanosine 5’-triphosphate (ara-GTP) in human peripheral blood mononuclear cells (PBMC). The determination of ara-GTP in PBMC was validated using a standard addition method with the human Tlymphoblastoid cell line as an alternative blank matrix. Ara-GTP was extracted with methanol/250 mmol/L ammonium carbonate solution (7/3, v/v) from the cells at a density of 106 cells per 0.5 mL. Extracts were subjected to LC-MS/ MS using a TurboIon spray interface and selected reaction monitoring with the transitions of m/z 524 to m/z 152 for quantitation. Endogenous guanosine triphosphate in the extract was used as an internal standard. Separation of the analytes was achieved on a porous graphitic carbon column (100 mm length × 2.1 mm i.d., 5 μm particle size) by isocratic elution with 250 mmol/L ammonium carbonate buffer (pH 9.5)/water/acetonitrile (40/51.5/8.5, v/v/v) at a flow rate of 0.2 mL/min. The method was validated in the range of 2–250 pg/mL. The pharmacokinetic profile of ara-GTP in PBMC in a Phase I clinical study of nelarabine in relapsed or refractory T-ALL/T-LBL patients was successfully determined using this method.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
Leave Your Message 24x7