A Survey of Viral-bacterial Co-infection in Respiratory Samples Using Multiplex Real Time-PCR | OMICS International| Abstract
ISSN: 2332-0877

Journal of Infectious Diseases & Therapy
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
  • Research Article   
  • J Infect Dis Ther 2019, Vol 7(2): 400
  • DOI: 10.4172/2332-0877.1000400

A Survey of Viral-bacterial Co-infection in Respiratory Samples Using Multiplex Real Time-PCR

Vijay Singh*, Jairus Reddy and John Granger
Department of Molecular Diagnostics, HealthTrackRx, Denton, Texas, USA
*Corresponding Author : Vijay Singh, Department of Molecular Diagnostics, HealthTrackRx, Denton, Texas, USA, Tel: +1940-383-2223, Email: [email protected]

Received Date: Apr 01, 2019 / Accepted Date: Apr 21, 2019 / Published Date: Apr 26, 2019


Influenza and non-Influenza virus related respiratory tract infections are amongst the most common reasons reported for physician visits and hospitalizations, globally. The economic burden associated with these viral infections, within the United States, are upwards of $50 billion annually. Concomitant, or subsequent, co-infections of the respiratory tract by pneumonia-causing pathogenic bacteria, like Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis and Staphylococcus aureus, are often observed in patients suffering from these respiratory viral infections and contribute to significant co-morbidity and mortality. In the present study, respiratory swabs from patients were tested for the presence of viral and bacterial pathogens, by Real-Time PCR, employing  the Thermo-Fisher Open Array® platform. Amongst the 5793 samples tested (Dec 2017-Dec 2018), 2357 (40.68%) tested positive for the presence of Influenza and Non-Influenza viral infections. Out of these positive  patient samples, 1175 (49.85%) also tested positive for the presence of one or more pneumonia-causing bacterial species. The co-infection data obtained was distributed on the basis of age. Interestingly, nearly 30% of the cases from the younger patient subset (<1 year old and 1-15 years old) were found to be co-infected with S. pneumoniae, H. influenzae and M. catarrhalis within the same sample. In addition, infant (<1 year old) patient viral positive samples, displayed higher than previously reported levels of M. catarrhalis co-infections (72.08%). With the ever-increasing popularity of point of care testing, this study demonstrates the importance and efficacy of a rapid, comprehensive multiplex-PCR, syndromic-panel approach to respiratory disease diagnostics, assisting clinicians to make better, and timely, decisions, potentially leading to improved patient care and outcomes.

Keywords: Influenza; Co-infections; Influenza like illness; Respiratory virus; Respiratory infection

Citation: Singh V, Reddy J, GrangerJ (2019) A Survey of Viral-bacterial Co-infection in Respiratory Samples Using Multiplex Real Time-PCR . J Infect Dis Ther 7: 400. Doi: 10.4172/2332-0877.1000400

Copyright: © 2019 Singh V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Select your language of interest to view the total content in your interested language

Post Your Comment Citation
Share This Article
Recommended Conferences
Article Usage
  • Total views: 1870
  • [From(publication date): 0-0 - Mar 01, 2021]
  • Breakdown by view type
  • HTML page views: 1715
  • PDF downloads: 155