Characterization of GA3 Insensitive Reduced Height Mutant of Emmer Wheat Var. NP200 (Triticum Dicoccum)
Received Date: May 07, 2014 / Accepted Date: Jun 19, 2014 / Published Date: Jun 21, 2014
Abstract
A γ-ray induced semi-dwarf mutant was obtained in tall emmer wheat variety NP200. The mutant was insensitive to externally applied gibberellin. An allele specific marker for dwarfing gene Rht-B1b was used to check the status of dwarfing gene in the mutant, semi dwarf and tall emmer and semi dwarf durum varieties. The primer showed amplification of Rht-B1b gene in semi dwarf durum and emmer varieties. The parent NP200 showed presence of wild type allele (Rht-B1a) with the primer pair BF-WR1. All semi dwarf emmer varieties showed a band of 237bp with primer pair BF-MR1showed presence of Rht-B1b. However, the mutant showed absence of amplification for both Rht-B1a and Rht-B1b alleles with respective primer pairs. The results indicated that the reduced height mutant carried a mutation different from the known allele Rht-B1b.
Keywords: Triticum dicoccum , GA3 insensitivity, Reduced height mutant, Rht genes, Yielding ability, Allele specific primer
Citation: Bakshi S, Nayeem KA, Bhagwat SG, Shitre A and Das BK (2014) Characterization of GA3 Insensitive Reduced Height Mutant of Emmer Wheat Var. NP200 (Triticum Dicoccum). Adv Crop Sci Tech 2:132. Doi: 10.4172/2329-8863.1000132
Copyright: © 2014 Suman Bakshi, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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