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Characterization of GA3 Insensitive Reduced Height Mutant of Emmer Wheat Var. NP200 (Triticum Dicoccum) | OMICS International | Abstract
ISSN: 2329-8863

Advances in Crop Science and Technology
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Research Article

Characterization of GA3 Insensitive Reduced Height Mutant of Emmer Wheat Var. NP200 (Triticum Dicoccum)

Suman Bakshi1*, Nayeem KA2, Bhagwat SG1, Shitre A1 and Das BK1
1Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai-400 085, India
2Indian Agricultural Research Institute, Regional Station, Wellington-643231, The Nilgiris, Tamil Nadu, India
Corresponding Author : Suman Bakshi
Nuclear Agriculture and Biotechnology Division
Bhabha Atomic Research Centre
Trombay, Mumbai-400 085, India
Tel: 0022-25592331
E-mail: [email protected]
Received May 07, 2014; Accepted Jun 19, 2014;Published June 21, 2014
Citation: Bakshi S, Nayeem KA, Bhagwat SG, Shitre A and Das BK (2014) Characterization of GA3 Insensitive Reduced Height Mutant of Emmer Wheat Var. NP200 (Triticum Dicoccum). Adv Crop Sci Tech 2:132. doi: 10.4172/2329-8863.1000132
Copyright: © 2014 Suman Bakshi, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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A γ-ray induced semi-dwarf mutant was obtained in tall emmer wheat variety NP200. The mutant was insensitive to externally applied gibberellin. An allele specific marker for dwarfing gene Rht-B1b was used to check the status of dwarfing gene in the mutant, semi dwarf and tall emmer and semi dwarf durum varieties. The primer showed amplification of Rht-B1b gene in semi dwarf durum and emmer varieties. The parent NP200 showed presence of wild type allele (Rht-B1a) with the primer pair BF-WR1. All semi dwarf emmer varieties showed a band of 237bp with primer pair BF-MR1showed presence of Rht-B1b. However, the mutant showed absence of amplification for both Rht-B1a and Rht-B1b alleles with respective primer pairs. The results indicated that the reduced height mutant carried a mutation different from the known allele Rht-B1b.


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